The WHISPER 20 SPD method

EVOSEP+ METHOD

ULTRA-SENSITIVE ROUTINE APPLICATIONS

Whisper 20 SPD features a longer gradient in a series of methods developed with Whisper Flow Technology.

It has a gradient flow of 100 nl/min with a 60 minutes method duration – delivered robustly by standard Evosep One hardware.

The method is ideal for multiplexed single-cell workflows and for analysis of samples, where material is limited, i.e. from laser-capture micro-dissected tissue samples.

Webinar: Whisper is here

Learn more about the advantages of the pioneering Whisper™ flow technology in our webinar hosted by Nicolai Bach, Head of Applications at Evosep, from December 16th 2021. 

A LONGER WHISPER METHOD FOR INCREASED PROTEOME COVERAGE

The Whisper 20 SPD is designed for our EV1112 column and EV1111 fused silica emitter. It runs with a gradient flow of 100 nl/min and provides sharp peaks with a median FWHM of 5.3 seconds.

It is part of our dynamic beta environment and updates may be implemented for improved performance.

 ULTRA SENSITIVE DIA ANALYSIS

Data-independent analysis is ideal for sensititve proteome analysis. Using a DIA Hybrid library, almost 2000 proteins are identified from only 1 ng of peptide starting material. 70% of these proteins are quantified with a coefficient of variation below 20%.

This percentage is increased with higher peptide loads. Diluted HeLa (Pierce) were used and data analyzed with Spectronaut.

REPRODUCIBLE RETENTION TIMES ALL THE TIME

Retention time reproducibility is a key value when developing new methods.

We monitored the stability in retention time of two peptides across 15 consecutive injections and found the standard deviation to be 10 and 15 seconds respectively.

Webinar: The launch in 2020

The Whisper™ flow technology was first launched on December 15th 2020. In this webinar, hosted by Nicolai Bache, Head of Applications at Evosep, you can learn more about the principles and advantages of the pioneering Whisper™ flow technology.

Additionally, Andreas-David Brunner from the Mann group at the Max Planck Institute of Biochemistry presents how he has implemented a nearly loss-less sample preparation strategy with the Whisper methods and diaPASEF on the timsTOF SCP mass spectrometer to analyze single-cell proteomics.

Get well started with Whisper

We are proud to introduce our new Whisper methods and would like you to get started well. Here you can find a step-by-step guide describing how to connect your column and emitter to obtain optimal peak shapes and thereby the best chromatographic performance.

Download guide

Sign up to our WHisper user group

Whisper is part of our dynamic environment, where updates are to be expected. Sign up here to get notified with relevant information.

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DOWNLOAD OUR MATERIAL

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ULTRA-SENSITIVE AND STABLE PERFORMANCE WITH WHIsPER FLOW TECHNOLOGY
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Whisper™ step-by-step guide
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Column ovens for Evosep performance columns
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columns and emitters overview
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EVOSEP ONE: A GRADIENT OFF-SET FOCUSING UHPLC INSTRUMENT FOR ROBUST AND HIGH THROUGHPUT PROTEOMICS

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