Whisper 20 SPD Beta
This EVOSEP+ method is ideal for ultra-sensitive applications, where starting material is limited.
ULTRA-SENSITIVE ROUTINE APPLICATIONS
Whisper 20SPD beta features a longer gradient in a series of methods developed with Whisper Flow Technology.
It has a gradient flow of 100 nl/min with a 60 minutes method duration – delivered robustly by standard Evosep One hardware.
Webinar: Whisper is coming
Learn more about the advantages of the pioneering Whisper™ flow technology in our webinar hosted by Nicolai Bach, Head of Applications at Evosep, from December 15th.
A LONGER WHISPER METHOD FOR INCREASED PROTEOME COVERAGE
The Whisper 20SPD Beta is designed for our EV-1112 column and EV-1111 fused silica emitter. It runs with a gradient flow of 100 nl/min and provides sharp peaks with a median FWHM of 5.3 seconds.
It is part of our dynamic beta environment and updates may be implemented for improved performance.
ULTRA SENSITIVE DIA ANALYSIS
Data-independent analysis is ideal for sensititve proteome analysis. Using a DIA Hybrid library, almost 2000 proteins are identified from only 1 ng of peptide starting material. 70% of these proteins are quantified with a coefficient of variation below 20%.
This percentage is increased with higher peptide loads. Diluted HeLa (Pierce) were used and data analyzed with Spectronaut.
REPRODUCIBLE RETENTION TIMES ALL THE TIME
Retention time reproducibility is a key value when developing new methods.
We monitored the stability in retention time of two peptides across 15 consecutive injections and found the standard deviation to be 10 and 15 seconds respectively.
“Single cell proteomics is becoming a practical reality and we are now able to routinely measure large cohorts of single cell proteomes to reveal new biological information. The Evosep One has been pivotal to scale this robustly to thousands of real single cell measurements, making it an ideal and standardized platform for future biomarker discovery work.”,
Professor Matthias Mann, Max-Planck Institute of Biochemistry,
Proteomics and Signal Transduction
Get well started with Whisper
We are proud to introduce our new Whisper methods and would like you to get started well. Here you can find a step-by-step guide describing how to connect your column and emitter to obtain optimal peak shapes and thereby the best chromatographic performance.
Sign up to our WHisper (beta) user group
Whisper is part of our dynamic beta environment, where updates are to be expected. Sign up here to get notified with relevant information.
explore all available methods with evosep+
Find the method fitting your research need and get inspired with data from Evosep users.
The extended method
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Maximum proteome coverage in a single-shot analysis with extended gradient duration.
The High organic method
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This EVOSEP+ method enables small-molecules analysis with significantly more hydrophobic targets.
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40SPD beta
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The ideal choice to achieve robust, very low nanoflow with ultra-sensitive single-cell analysis.
The lipidomics method
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In development
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ULTRA-SENSITIVE AND STABLE PERFORMANCE WITH WHIsPER FLOW TECHNOLOGY
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Whisper™ step-by-step guide
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Column ovens for Evosep performance columns
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