Whisper 20 SPD Beta

This EVOSEP+ method is ideal for ultra-sensitive applications, where starting material is limited.  

ULTRA-SENSITIVE ROUTINE APPLICATIONS

Whisper 20SPD beta features a longer gradient in a series of methods developed with Whisper Flow Technology.

It has a gradient flow of 100 nl/min with a 60 minutes method duration – delivered robustly by standard Evosep One hardware.

Webinar: Whisper is coming

Learn more about the advantages of the pioneering Whisper™ flow technology in our webinar hosted by Nicolai Bach, Head of Applications at Evosep, from December 15th. 

A LONGER WHISPER METHOD FOR INCREASED PROTEOME COVERAGE

The Whisper 20SPD Beta is designed for our EV-1112 column and EV-1111 fused silica emitter. It runs with a gradient flow of 100 nl/min and provides sharp peaks with a median FWHM of 5.3 seconds.

It is part of our dynamic beta environment and updates may be implemented for improved performance.

 ULTRA SENSITIVE DIA ANALYSIS

Data-independent analysis is ideal for sensititve proteome analysis. Using a DIA Hybrid library, almost 2000 proteins are identified from only 1 ng of peptide starting material. 70% of these proteins are quantified with a coefficient of variation below 20%.

This percentage is increased with higher peptide loads. Diluted HeLa (Pierce) were used and data analyzed with Spectronaut.

REPRODUCIBLE RETENTION TIMES ALL THE TIME

Retention time reproducibility is a key value when developing new methods.

We monitored the stability in retention time of two peptides across 15 consecutive injections and found the standard deviation to be 10 and 15 seconds respectively.

“Single cell proteomics is becoming a practical reality and we are now able to routinely measure large cohorts of single cell proteomes to reveal new biological information. The Evosep One has been pivotal to scale this robustly to thousands of real single cell measurements, making it an ideal and standardized platform for future biomarker discovery work.”,

Professor Matthias Mann, Max-Planck Institute of Biochemistry,
Proteomics and Signal Transduction

 

Get well started with Whisper

We are proud to introduce our new Whisper methods and would like you to get started well. Here you can find a step-by-step guide describing how to connect your column and emitter to obtain optimal peak shapes and thereby the best chromatographic performance.

Download guide

Sign up to our WHisper (beta) user group

Whisper is part of our dynamic beta environment, where updates are to be expected. Sign up here to get notified with relevant information.

explore all available methods with evosep+

Find the method fitting your research need and get inspired with data from Evosep users.

The extended method 

EVOSEP+ method

Maximum proteome coverage in a single-shot analysis with extended gradient duration.

LEARN MORE

The High organic method

EVOSEP+ method

This EVOSEP+ method enables small-molecules analysis with significantly more hydrophobic targets.

LEARN MORE

Whisper
40SPD beta

EVOSEP+ method

The ideal choice to achieve robust, very low nanoflow with ultra-sensitive single-cell analysis.

LEARN MORE

Whisper
20SPD beta

EVOSEP+ method

A longer method for sensitive nanoflow applications.

LEARN MORE

The lipidomics method

EVOSEP+ method

In development

DOWNLOAD OUR MATERIAL

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ULTRA-SENSITIVE AND STABLE PERFORMANCE WITH WHIsPER FLOW TECHNOLOGY
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Whisper™ step-by-step guide
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Column ovens for Evosep performance columns
BROCHURE
columns and emitters overview
tech note
EVOSEP ONE: A GRADIENT OFF-SET FOCUSING UHPLC INSTRUMENT FOR ROBUST AND HIGH THROUGHPUT PROTEOMICS