The High organic method

EVOSEP+ METHOD

small-molecules analysis with significantly more hydrophobic targets

This EVOSEP+ method enables small-molecules analysis with significantly more hydrophobic targets.

It is specifically developed to detect stanozolol in equine hair and urine. 

Large scale doping analysis

Anabolic androgenic steroids doping is still a significant problem in horse racing and sport testing laboratories run tens of thousands of samples per year. They are continuously seeking for higher sensitivity and robustness to detect trace levels of performance enhancing drugs.

The High Organic method is specifically developed to detect stanozolol; a possible performance enhancing synthetic AAS, and its phase I metabolites in challenging matrices such as horse urine and hair.

It enables small-molecules analysis of significantly more hydrophobic targets. Thus, the analytes are sequentially eluted of the Evotips with up to 100% acetronitrile with a throughput of 100 samples per day.

Exceptional retention time reproducibility

The method has a throughput of 100 samples per day and is specifically designed for our EV1064 Endurance column.

The run-to-run retention time stability over 30 injections with a standard mixture demonstrated excellent reproducibility with a standard deviation of just 1.1 seconds on average.

User talk

ULTRA-SENSITIVE DOPING CONTROL ANALYSIS OF THE MOST NOTORIOUS SYNTHETIC ANABOLIC STEROID

The High Organic method is developed in collaboration with GIE-LCH, Laboratoire Des Courses Hippiques, specifically to enable small-molecules analysis.

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The High organic method

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This EVOSEP+ method enables small-molecules analysis with significantly more hydrophobic targets.

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DOWNLOAD OUR MATERIAL

Application note
A specialized separation method for hydrophobic targets with the High Organic method
Application note
Extended detection of anabolics misuse in high-throughput doping controls with the Evosep One
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columns and emitters overview
Application Note
A specialized method for maximum proteome coverage with the Extended method

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