The High organic method
EVOSEP+ METHOD
small-molecules analysis with significantly more hydrophobic targets
This EVOSEP+ method enables small-molecules analysis with significantly more hydrophobic targets.
It is specifically developed to detect stanozolol in equine hair and urine.
Large scale doping analysis
Anabolic androgenic steroids doping is still a significant problem in horse racing and sport testing laboratories run tens of thousands of samples per year. They are continuously seeking for higher sensitivity and robustness to detect trace levels of performance enhancing drugs.
The High Organic method is specifically developed to detect stanozolol; a possible performance enhancing synthetic AAS, and its phase I metabolites in challenging matrices such as horse urine and hair.
It enables small-molecules analysis of significantly more hydrophobic targets. Thus, the analytes are sequentially eluted of the Evotips with up to 100% acetronitrile with a throughput of 100 samples per day.
Targeted analysis of stanozolol
The High Organic method is developed for targeted analysis of stanozolol in equine urine and hair.
Negatively tested equine urine was spiked with 16β-hydroxy-stanozolol with 10 and 100 pg/mL respectively.
The signal was absent in the blank samples, whereas spiked urine extracts showed efficient signal of fragment ion chromatograms of 16β-OH-stanozolol.
Exceptional retention time reproducibility
The method has a throughput of 100 samples per day and is specifically designed for our EV1064 Endurance column.
The run-to-run retention time stability over 30 injections with a standard mixture demonstrated excellent reproducibility with a standard deviation of just 1.1 seconds on average.
User talk
ULTRA-SENSITIVE DOPING CONTROL ANALYSIS OF THE MOST NOTORIOUS SYNTHETIC ANABOLIC STEROID
The High Organic method is developed in collaboration with GIE-LCH, Laboratoire Des Courses Hippiques, specifically to enable small-molecules analysis.
In this webinar, Vivian Delcourt presents the method using the synthetis analbolic steroid “stanozolol” as a reference.
The application allowed the expanded detection window in complex matrices such as equine urine and hair and will constitute new landmarks for doping control laboratories.
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The High organic method
EVOSEP+ method
This EVOSEP+ method enables small-molecules analysis with significantly more hydrophobic targets.
The extended method
EVOSEP+ method
Maximum proteome coverage in a single-shot analysis with extended gradient duration.
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Application note
A specialized separation method for hydrophobic targets with the High Organic method
Application note
Extended detection of anabolics misuse in high-throughput doping controls with the Evosep One
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columns and emitters overview
Application Note
A specialized method for maximum proteome coverage with the Extended method
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