70th ASMS conference

Join Evosep at ASMS 2022

June 5-9 2022  I  Minneapolis Convention Center

Meet Evosep at the orange corner

We are more than ready for this year’s ASMS, the largest annual gathering of mass spectrometry scientists. We are excited once again meet up with minds a like and to learn more about the current needs and research being published in the community. 

All week you can meet us at our orange Evosep corner and we would love to meet you and learn more about you and your current research. We are also more than happy to introduce you to our technology. We will also like to invite you to join our hospitality suite – and we promise, you will not be dissapointed about this year’s theme!

Learn more about all our activities at this year’s ASMS below

Scientific presence

Our booth is not the only place to learn more about Evosep and the many research posibilities available.  We are excited to advocate several interesting customer talks during this year’s ASMS week.

Learn more about them below and find the full list of all abstracts featuring Evosep One. 

Evosep Breakfast seminar

High throughput proteomics with a focus on automation

Monday June 6th at 7:15 am CS

Join us for breakfast and get up to speed with high-throughput proteomics and learn why an effective sample preparation workflow is pivotal to succeed. We will discuss easy-to-implement automated solutions to facilitate high-throughput applications.

Speakers:

High throughput proteomics with a complete and automated sample preperation workflow

Talk by Jesper V. Olsen, Vice Director and Professor at Novo Nordisk Foundation Center for Protein Research, University of Copenhagen

In this breakfast seminar, I will present a fully automated end-to-end proteomics sample preparation workflow from cell lysate to tryptic peptides on Evotips in two and a half hours or phosphopeptide enriched samples in about 5 hours.

The entire workflow is performed on the Opentrons-2 robot and includes a full protein aggregation capture (PAC) trypsin digestion using magnetic beads and automated loading of (phospho)-peptides on Evotips with an integrated prototype loading module from Evosep. This enables a complete hands-off workflow for processing 48 or 96 samples in parallel, ready for injection on the Evosep One in a just a few hours.

Achieving high-throughput HCP analysis for industrial enzyme manufacturing

Talk by Anders Giessing, Senior Scientist at Novozymes

Here we present a High-Throughput Host Cell Protein Analysis workflow build to support an industrial protein MS core facility. Analytical and data analysis solutions are combined to meet a demand to handle high sample and sequence complexity. The workflow is based on an Evosep-timsTOF Pro LC-MS/MS setup combined with a customized Gendata data analysis platform.

Evosep user appreciation event: Buffet and Science

Meet other users and get the latest tips and tricks

Saturday June 4th at 5:30 pm  I  Rock bottom restaurant & brewery, 825 Hennepin Ave Suite 125 Minneapolis, MN 02116 – 15 min walk from the conference – see directions

We are excited to host an Evosep One user meeting at this year’s ASMS and we hope you would like to spend a few informal hours with us. We will make sure you get some delicious refreshments while getting you up to speed with different topics we feel are relevant to discuss and you can meet other users and learn about their research.

And, if you are curious about increased throughput and sensitivity, you should definitely join us. 

High throughput Plasma proteomics by DIA-MS: May the best method win

Talk by Annie Moradian, PhD, Lead Biomedical Scientist at Precision Biomarker Labs, CEDARS-SINAI

The goal of this project is to assess DIA of plasma samples by varying gradient times of an Evosep one and DIA isolation window size of an Exploris 480 Orbitrap to determine the best method that maximizes protein identification in the shortest amount of time with high reproducibility.

Method Optimization of a New Automated Platform for Proteome-Wide Structural Biology

Talk by Danté Johnson, PhD Candiate, University of Maryland

Platform Incubator with Movable XY stage (PIXY) is an automated system developed to characterize cellular proteins using hydroxyl radical protein footprinting method Fast Photochemical Oxidation of Proteins (FPOP). Our optimized workflow of In-cell FPOP coupled with 2D offline fractionation and Evosep One-mediated LC-MS/MS results in increased diversity of FPOP modified peptides detected with high throughput.

Chromatographic improvement of the 30SPD method with application to spatially resolved liver proteomics

Talk Marvin Thielert, PhD Graduate Student at Max Planck Institute of Biochemistry

We used the newly introduced performance column to enhance proteome coverage for the 30SPD and 15SPD method in DDA and DIA and we were able to reach a proteomic depth of up to 8,000 protein groups. Using our recent advancement „Deep Visual Proteomics“ that combines microscopy, AI-based image segmentation and ultra-high sensitive mass spectrometry, we spatially resolve the architecture of liver tissue, with a plethora of applications in clinical proteomics of the future. With the improved 30SPD method, we were able to quantify the proteome of an equivalent of two hepatocytes from mouse liver tissue at a depth of 1,200 protein groups.

evosep-logo

Robust and High-Throughput Single Cell Analysis with the Evosep One

Talk by Dorte Bekker-Jensen, Scientist at Evosep

At this user meeting, we will present the latest improvements to our Whisper workflows for high-sensitivity single cell analysis. This includes improved chromatographic performance, where slimmer peaks result in more than 50% higher peptide coverage. Additionally, we will discuss how to accelerate the sample throughput to enable analysis of larger cohorts of single cells.

 

evosep-logo

Nicolai Bache, Head of Applications at Evosep (host) 

AGILENT BREAKFAST SEMINAR

OMICS REMIX:ULTRA-HIGH SENSITIVITY FOR TARGETED PROTEOMIC USING EVOSEP-MRM AT SINGLE CELL LEVEL

Talk by Nicolai Bache, Head of Applications at Evosep

Join this event to explore the power of single cell proteomics. The Evosep One is a powerful and standardized platform for high sensitivity work including single cell proteomics. The robustness and throughput of the Whisper methods on the Evosep One combined with the sensitivity of the Agilent 6495C triple quadrupole support targeted analysis of proteomes at the single cell level. We demonstrated this by targeting selected peptides in a complex background of sub nanogram material combined with dilution curves spanning several orders of magnitude. To ensure seamless integration of the EvosepOne with the Agilent 6495C triple quadrupole, we have developed a native driver for MassHunter and a robust solution for the Agilent nanoflow ion source.

When and where: Wednesday June 8th at 7:15 am CST  I  Ballroom ABC

This event is hosted by

BRUKER USER MEETING 

EXCEED SYMPOSIA: ENHANCING WORKFLOW CAPABILITIES FOR TRANSLATIONAL MASS SPECTROMETRY

Meet Evosep at the Bruker eXceed symposia.

When and where: Sunday June 5th

This event is hosted by

TALK 

FULLY AUTOMATED END-TO-END WORKFLOW FOR HIGH-THROUGHPUT ANALYSIS OF PROTEOMES AND PHOSPHOPROTEOMES FROM LOW SAMPLE AMOUNTS

Talk by Florian Harking, Ph.D. student at NNF Center for Protein Research, University of Copenhagen

Here, we present a fully automated end-to-end proteomics sample preparation workflow from cell lysate to purified tryptic peptides in less than 2 hours and with the option to include an enrichment step of phosphopeptides in additional 2 hours. The entire workflow is performed on the Opentrons-2 robot and includes a full PAC digestion and automated loading of (phospho)-peptides on Evotips with an integrated loading module from Evosep (prototype) enabling a hands-off workflow all the way from lysate to samples ready for injection on the Evosep One in a just a few hours.We optimized the protocol for speed and simplicity and focused on low amounts in the range of less than 200 ug protein starting amount for phosphoproteomics.

When and where: Thursday June 9th Session: High Throughput MS

Hospitality suite

Need a scientific break? 

We got you covered. Stop by our hospitality suite for a drink and enjoy this year’s theme, some very cool giveaways and fun activities.

 June 6 – 8  at 8:00 pm – 11:00 pm  I   Room: Directors Row 1

News

evosep-logo

(April 2022) EVOSEP INTRODUCES NEW EVOTIP PURE FOR IMPROVED PERFORMANCE, ESPECIALLY FOR HIGH SENSITIVITY WORKFLOWS

Leipzig – EuPA 2022. Today, at the XIV Annual Congress of the European Proteomic Association, Evosep introduces a new and vastly improved generation of Evotips, called Evotip Pure™.

Abstracts

This year you can explore a lot of new research featuring Evosep One at ASMS. Below you can find a complete list of all the submitted abstract. Use the search function to filter based on author, organization or topic.

TitleSummary
MP 304 4D proteomic profiling of 200 individuals: a high throughput workflow from sample preparation, dia-PASER-MS to 4D data processing to profilingTalk by Qin Fu, Senior Project Advisor at Cedars-Sinai Medical Center, Smidt Heart Institute. (Description) Date: Monday, June 6. Session: Ion Mobility: Applications I.
TP 484 A deeper look at carrier proteome effects for single-cell proteomicsTalk by Zilu Ye, Postdoc at University of Copenhagen (CPR). (Description) Date: Tuesday, June 7. Session: Single Cell MS.
MP 400 A mass spectrometry-based proteomics approach for FFPE laser-capture microdissected renal tissue analysis focused on Monoclonal Gammopathy of Clinical SignificanceTalk by Marta Vilaseca, Core Facility Manager at Institute for Research in Biomedicine (IRB Barcelona). (Description) Date: Monday, June 6. Session: Proteomics: Clinical Applications.
WP 041 An unbiased multi-omics approach for the detection of pancreatic cancer biomarkers utilizing ion-mobility mass spectrometry and nano-particle based Proteograph technologyTalk by Kavya Swaminathan, Senior Scientist at PrognomiQ Inc. (Description) Date: Wednesday, June 8. Session: Biomarkers: Discovery I.
WP 127 Application of In-Cell Fast Photochemical Oxidation of Proteins for the Study of OrganoidsTalk by Raquel Shortt, Ph.D. student at University of Maryland. (Description) Date: Wednesday, June 8. Session: Covalent Labeling and Chemical Crosslinking I.
TP 233 CCS-Aware DIA Data AnalysisTalk by Robin Park, Director of Bioinformatics at Bruker Daltonics LLC. (Description) Date: Tuesday, June 7. Session: Informatics: Peptide ID and Quantification.
MP 070 Data-independent acquisition proteomics with super-resolution Orbitrap mass spectrometryTalk by Sophia Steigerwald, Ph.D. student at Max Planck Institute of Biochemistry, Planegg. (Description) Date: Monday, June 6. Session: Data-Independent Acquisition.
ThP 275 Exploring linear sequence determinants of inferred Collisional Cross-Sections of unmodified and phosphorylated peptides in an Orbitrap Mass AnalyzerTalk by Ulises Hernandez Guzman, Assistant Research Scientist at Novo Nordisk Foundation for Protein Research, Copenhagen. (Description) Date: Thursday, June 9. Session: Ion Mobility: Fundamentals.
WP 248 High throughput Plasma proteomics by DIA-MS: May the best method winTalk by Annie Moradian, Lead Biomedical Scientist at Precision Biomarker Laboratories, Cedars-Sinai Medical Center. (Description) Date: Wednesday, June 8. Session: High Throughput MS.
WP 010 High-throughput DIA-MS proteomics combined with a novel deep learning approach identifies many more analytes than current benchmark data analysis strategiesTalk by Brian Maples, CTO at Naring Health. (Description) Date: Wednesday, June 8. Session: Artificial Intelligence in MS Instrumentation and Applications
WP 121 Identifying reactive cysteines of gene regulatory proteins through cellular fractionationTalk by Daniele Canzani, Research Scientist at Talus Bioscience. (Description) Date: Wednesday, June 8. Session: Covalent Labeling and Chemical Crosslinking I.
MP 077 Improved data-independent acquisition (DIA) and data-dependent acquisition (DDA) performance on low-level proteomic samples using a novel Zeno trapTalk by Ihor Batruch, Technical Product Manager at SCIEX. (Description) Date: Monday, June 6. Session: Data-Independent Acquisition.
ThP 054 Integration of Fluorescence-based Dosimetry to the IC-FPOP PIXY Platform Increases Quantitative Power for Protein Folding StudiesTalk by Dante Johnson, Ph.D. student at University of Maryland, Baltimore School of Pharmacy. (Description) Date: Thursday, June 9. Session: Biomolecular Structure Analysis: Chemical Crosslinking and Covalent Labeling
ThP 388 Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexingTalk by Claire Koenig, Ph.D. fellow at Novo Nordisk Foundation Center for Protein Research Copenhagen. (Description) Date: Thursday, June 9. Session: Phosphopeptides: Quantitative Analysis.
TP 495 Optimizing experimental conditions for enhancing sensitivity of single cell proteomicsTalk by Dong-Gi Mun, Ph.D., Mitochondrial Care Research Center – Mayo Clinic. (Description) Date: Tuesday, June 7. Session: Single Cell MS.
TP 497 Patch-Clamp Single Cell Proteomics: a multiplexed approach for determining changes in amygdala neurons after chronic ethanol exposureTalk by Jolene Diedrich, Scientist at The Scripps Research Institute. (Description) Date: Tuesday, June 7. Session: Single Cell MS.
ThP 011 Proteome profiling of plasma microvesicles isolated from patients with systemic sclerodermaTalk by Ole Østergaard, Postdoc at University of Copenhagen (Description) Date: Thursday, June 9. Session: Biomarkers: Discovery II
ThP 386 Rapid and in-depth coverage of the (phospho-)proteome with deep libraries and optimal window design for dia-PASEFTalk by Patricia Skowronek, Ph.D. student at Max Planck Institute Planegg (Description) Date: Thursday, June 9. Session: Phosphopeptides: Quantitative Analysis
ThP 385 Simultaneous targeted and discovery phosphoproteomics of cell signaling pathways using novel hybrid-DIA acquisition strategyTalk by Ana Del Val, Postdoc at Novo Nordisk Foundation Center for Protein Research Copenhagen. (Description) Date: Thursday, June 9. Session: Phosphopeptides: Quantitative Analysis
Strategies for the assessment of data independent acquisition method optimization for complex protein digestsTalk by Deanna Plubell, Ph.D. student at University of Washington. (Description) Date: Thursday, June 9. Session: ThOD am: Informatics: Data-Independent Acquisition and Multiplexing.
The Need for Speed: Compressed Sensing With Generative Models Enables Ultra High-Throughput DIA Mass SpectrometryTalk by William Fondrie, Senior Data Scientist at Talus Bioscience (Description) Date: Tuesday, June 7 Session: TOD pm: Artificial Intelligence in MS Instrumentation and Applications
MP 079 Using quantitative benchmarks to assess accuracy and ensure consistency in label-free data independent mass spectrometry studies at scaleTalk by Lindsay Pino, Co-founder and CTO at Talus Bio Science (Description) Date: Monday, June 6 Session: Data-Independent Acquisition
TP 343 A complete and automated samle preparation strategy for high-throughput and standardized proteomics applied to a cohort of patient plasma samplesTalk by Dorte Bekker-Jensen, Senior Scientist at Evosep (Description) Date: Tuesday June 7 Session: Biomarkers: LC/MS: Sample Preparation
WP 059 An Easier, More Robust, and Faster Low-flow Quantitative Proteomics ApproachTalk by Wendi Hale, Ph.D., LC/MS Applications Scientist at Agilent (Description) Date: Wednesday June 8 Session: Biomarkers: Quantitative Analysis I
ThP 038 Ultra-High Sensitivity for Targeted Mass Spectrometry ApplicationsTalk by Angela Mc Ardle, Senior scientist at Evosep (Description) Date: Thursday June 9 Session: Biomarkers: Quantitative Analysis II
WP 043 Development of a Semi-Automated Sample Loading Protocool for Robust and High Throughput Analysis on the Evosep OneTalk by Angela Mc Ardle, Senior scientist at Evosep (Description) Date: Wednesday June 8 Session: Biomarkers: Discovery I
MP 393 High-throughput automated Evosep-MRM workflow for glycated albumin quantification to facillitate the diagnosis of diabetes mellitusTalk by Bharath Kumar Raghuraman, Project scientist at Evosep (Description) Date: Monday June 6 Session: Proteomics: Clinical Applications.
WP 011 Ultrafast bacterial detection in urinary tract infections using high-throughput proteomics and artificial intelligenceTalk by Mickael Leclercq, research assistant at Centre de recherche du CHU de Québec-Université Laval (Description) Date: Wednesday June 8 Session: Artificial Intelligence in MS Instrumentation and Applications.
Fully automated end-to-end workflow for high-throughput analysis of proteomes and phosphoproteomes from low sample amountsTalk by Florian Harking, Ph.D. student at NNF Center for Protein Research, University of Copenhagen (Abstract) Date: Thursday June 9 Session: High Throughput MS

MEET THE TEAM

This year you can meet both representatives from our Applications-, Management-, Sales- and Product team. Learn a little bit about the team at ASMS here. 

Nicolai Bache

Nicolai Bache

Head of Applications

Dorte Bekker-Jensen

Dorte Bekker-Jensen

Senior Scientist

Michael BarRett Andersen

Michael BarRett Andersen

Head of Product Management

Erik Verschuuren

Erik Verschuuren

Head of Global Sales

Ole Vorm

Ole Vorm

Founder

Christian Ravnsborg

Christian Ravnsborg

CEO

Laurent Rieux

Laurent Rieux

Product Specialist

Thomas Carrell

Thomas Carrell

Sales Specialist

John Lindsay

John Lindsay

Sales Representative

Craig Bury

Craig Bury

Sales Representative

LET’S GET IN TOUCH

We are always open to answering questions and form new collaborations, so don’t hesitate to reach out. Fill out our contact form and we’ll do our very best to assist you.