71st ASMS conference
Evosep at ASMS 2023
Learn more about all our activities at this year’s ASMS below
Meet Evosep at the orange corner
We are eagerly looking forward to this year’s ASMS, the biggest annual congregation of scientists working on mass spectrometry. This year the conference is held in Houston, Texas and we cannot wait to meet your and share our latest projects with you.
You can find us throughout the week at our orange Evosep corner at booth #712, where we would be thrilled to connect with you and exchange information about your ongoing research. Furthermore, we are delighted to introduce you to new advancements of our technology, and additionally, we will also host our User Meeting, Breakfast Seminar and Hospitality Suite, where we guarantee that this year’s special theme will exceed your expectations!
Evosep user Meeting: Food for thought
Saturday, June 3, 2023 – 5.30 – 9 PM – True Anomaly Brewing CO – 2012 Dallas St, Houston
MEET OTHER USERS AND GET THE LATEST TIPS AND TRICKS
We are thrilled to announce our upcoming Evosep One user meeting during this year’s ASMS, and we would love to have you join us for a few informal hours of knowledge-sharing.
There will be delicious refreshments while getting you up to speed with different topics we feel are relevant to discuss and you can meet other users and learn about their research.
And, if you are curious about automated end-to-end workflows, you should definitely join us.
High-throughput analysis of deep plasma proteomes by enrichment of extracellular vesicles
Talk by PhD. Ole Østergaard, Proteomics Specialist at Novo Nordisk Foundation Center for Protein Research
Human plasma is an attractive source for protein biomarker discovery as it is in contact with essentially all organ systems in the human body and relatively easy to collect. However, plasma is also a challenging bio-specimen due to the high dynamic range of plasma proteins with quantification of typically only few hundred proteins in standard mass spectrometry (MS)-based proteomics workflow. Extracellular vesicles (EVs) are membrane-enclosed bodies shed constitutively from cells as part of both physiological and pathological processes. Enrichment of EVs from plasma followed by data-independent acquisition (DIA) MS-analysis using fast gradients on the Evosep One LC enables reproducible quantification of 10-fold more proteins from plasma including proteins associated with relevant pathology making this a powerful strategy in clinical proteomics.
Very large-scale cerebrospinal fluid proteomics for biomarker discovery in multiple sclerosis
Talk by Jakob Bader, PhD student, Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry
Proteomics in body fluids is challenged by the high range of protein abundances and the large cohorts required for biomarker studies. Here, we present a scalable and high-throughput mass spectrometry (MS) proteomics CSF workflow and validate it in a cohort of 6000 CSF samples from patients with various neurological conditions; detecting an average of 1300 proteins per sample. The system is built around the Evosep One chromatography system and the Bruker timsTOF Pro 2, utilizing a 21-minute gradient (60 samples per day) and data-independent acquisition (DIA) scheme. We characterize the robustness of the overall system, including on chromatography level. The large dataset obtained via this scalable workflow synergizes with machine learning to define markers for Multiple Sclerosis.
Nicolai Bache, Head of Applications at Evosep (host)
Evosep Breakfast Seminar
monday, june 5, 2023 – 7:00 – 8:15 AM – room 310BC – George R Brown Convention Center
standardizing high-throughput proteomics
Come and have breakfast with us as we dive into the world of high-throughput proteomics and discover the crucial role of automated end-to-end workflows.
Clinical proteomics workflows enabled by the Evosep system
Talk by Professor Matthias Mann, Max-Planck Institute of Biochemistry, Proteomics and Signal Transduction
Although the basic set up of mass spectrometry – based proteomics has essentially stayed the same over two decades, the capabilities of this technology have increased exponentially. Currently this manifests mainly in the areas of throughput, robustness and sensitivity, each of which the Evosep system has profoundly impacted. In our group, we have now nearly completely shifted to short gradients (typically 60 samples per day) and to commercial columns (typically Pepsep or IonOptiks but also uPAC). The shift from home built set ups to a more standardized format has tremendously accelerated our research without decreasing performance. It also coincides with increasingly faster MS instruments such as the Bruker, Thermo or SCIEX platforms. In turn, this has enabled us to tackle increasingly large-scale clinical studies, including thousands of plasma or CSF samples, some result of which will be described here. Furthermore, we routinely use the low flow Whisper gradients for single cell analysis or any other ultra-high sensitivity analyses. Our main focus now is the ‘Deep Visual Proteomics’ technology, where we combine AI based segmentation and classification of cells in tumor tissues with automated laser micro dissection and mass spectrometry.
Integration of chip-based sample preparation and high-throughput chromatography enables single-cell chemical proteomics
Talk by Claudia Ctortecka, Postdoc at Broad Institute of MIT and Harvard
Our most recent miniaturized single-cell proteomics (SCP) workflow integrates a dedicated proteoCHIP directly with the Evosep One. The nanoliter sample preparation with 40SPD and diaPASEF acquisition quantifies more than 3,000 proteins per single cell. These identifications are highly reproducible with cumulative missing values of ≤10% spanning over 4 orders of magnitude. This proteome depth is sufficient to represent dose dependent small-molecule effects and identifies targeted compound perturbations. Moreover, we demonstrate that even 80SPD can be effectively used for rapid screening while recovering 75% of protein identifications and biologically relevant targets.
Monday – wednesday, 5-7 June, 2023 – 8.00 – 11.00 PM
Need a scientific break?
We got you covered. Stop by our hospitality suite for a drink and enjoy this year’s theme, cool giveaways and fun activities.
Room 335B – Hilton Americas, Houston
Register here to our events at ASMS 2023
We cannot wait to be back and would love for you to join us!
ASMS 2023 highlights: Automating end-to-end workflows
June 15, 2023 I 4:00 PM CEST / 10:00 AM EDT
Get an overview of all the highlights of Evosep at ASMS 2023, including new research, talks and much more from the conference!
This year you can explore a lot of new research featuring Evosep One at ASMS. Below you can find a complete list of all the submitted abstract. Use the search function to filter based on author, organization or topic.
|Oral presentation – A generic loading strategy for automated sample loading of Evotips for robust and high throughput analysis on the Evosep One|
|TP 642 – Next-generation biotyping of microorganisms for assessment of antibiotics resistance and virulence factors|
|TP 152 – A complete, automated end-to-end workflow for high-throughput therapeutic drug monitoring using Evosep MRM|
|TP 566 – High throughput quantification of biologics PTM state by peptide mapping using refocussing chromatography and ZenoTOF mass spectrometry|
|WP 655 – Pipetting-free single cell analysis with the label-free proteoCHIP and the Evotip adapter for high sensitivity proteomics on the timsTOF SCP|
|TP 156 – High-throughput end-to-end automated Evosep-MRM workflow for the quantification of glycated albumin in diabetes|
|ThP 421 – A complete and automated end-to-end sample preparation strategy for high-throughput and standardized proteomics with high sensitivity|
|WP 656 – Pushing the boundaries for robust and high-throughput single cell analysis with Whisper Flow technology powered by dia-PASEF|
MEET THE TEAM
This year you can meet both representatives from our Applications-, Management-, Sales- and Product team.
Learn a little bit about the team at ASMS 2023 here.
Head of Applications
Michael BarRett Andersen
Head of Product Management
Head of Global Sales
Ole Bjeld Hørning
Head of Technology
Digital Marketing Specialist
(May 2023) EVOSEP AND CELLENION ENTER CO-MARKETING AGREEMENT TO BOOST SINGLE CELL PROTEOMICS
Singapore – AOHUPO (May 9th, 2023). Evosep is pleased to announce a new co-marketing partnership with Cellenion. This partnership will enable researchers to access a complete solution for single-cell proteomics, combining Evosep’s cutting-edge liquid chromatography technology with Cellenion’s advanced single-cell isolation and sample preparation platform.
(March 2023) Evosep and Resyn Biosciences partner to integrate their technologies into new clinical proteomics solutions
ReSyn Biosciences and Evosep have recently demonstrated several joint solutions thatintegrate automated sample purification with liquid chromatography coupled mass spectrometry.
(March 2023) Evosep ESTABLISHES CENTER OF EXCELLENCE FOR HIGH-THROUGHPUT AND STANDARDIZED PROTEOMICS
Evosep Biolabs will partner with key players in the proteomics area, execute end-to-end workflows on customer projects, remove barriers to entry by acting as an external, expert lab partner, as well as providing extra, ad-hoc capacity to established organizations.
(January 2023) EVOSEP RECEIVES STRATEGIC INVESTMENT FROM NOVO HOLDINGS A/S TO ACCELERATE ITS EFFORT ON CLINICAL PROTEOMICS
Odense, Denmark – Evosep Aps (January 9, 2023) – Evosep today announced that Novo Holdings A/S has become a minority shareholder in Evosep Aps with a US$ 40m investment to further Evosep’s technology and application portfolio for clinical proteomics.
(December 2022) Evosep adds automation to its standardized platform
Cancun, Mexico – HUPO 2022. Today, at the 21st Human Proteome Organization World Congress, Evosep launches new separation methods and a protocol for automated loading of Evotips enabling end-to-end high throughput proteomics workflows.
LET’S GET IN TOUCH
We are always open to answering questions and form new collaborations, so don’t hesitate to reach out. Fill out our contact form and we’ll do our very best to assist you.