The 300 samples per day method

STANDARD METHOD

ULTRA HIGH-THROUGHPUT ANALYSIS

The Evosep One is designed for high throughput and robustness, while maintaining sensitivity for proteomics applications.

The 300 SPD method is ideally suited for routine clinical analysis with high run-to-run reproducibility of just 0.24 seconds.

The HIGHEST CHROMATOGRAPHIC PERFORMANCE PER GRADIENT MINUTE

The 300 SPD method provides the highest peak capacity per minute among our standard methods. Peak capacity is the most common measure of chromatographic performance and is defined as the number of peaks, that can be resolved over a gradient.

For high throughput analysis of complex samples, highest peak capacity in the shortest time is a relevant measure. This is achieved by ultra fast methods employing short columns and high flow rates providing the best separation power per gradient minute made affordable by the low overhead time on the Evosep One.

Importantly, the narrower peaks greatly reduce redundant sampling thus suggesting that the sensitivity of the analysis is boosted by employing the faster standard methods.

High sequencing speed with modern mass spectrometers

The first peptides elute already after 40 seconds, and with a full elution window of 2.3 minutes, the effective gradient elution is 72%. The short overhead time of only 1.6 minutes allows for an ultra high throughput of 300 samples per day.

By utilizing the high sequencing speed on modern mass spectrometers, a high load identifies 3300 unique peptides corresponding to nearly 1000 proteins.

The five standard methods

With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You device how fast you want to go. 

Standard method

The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.

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Standard method

Excellent sequencing speed with most peptides per minute among our standard methods.

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Standard method

A great “in-between” method with run-to-run reproducibility of just one second.

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Standard method

The most popular method with an excellent balance of throughput and depth.

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Standard method

The longest method with a 44-minute gradient providing the best overall proteome coverage.

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Explore more

Do you want to know how our users are already taking advantage of the 300 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn More
Towards a Standardized Omics Platform with the 300 samples per day method2020This is the fastest of our standard proteomics methods with a throughput of 300 samples per day.

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The proteomics dilemma – High throughput analysis versus proteome depth2019With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis., , , ,

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Targeted assay development for quantifying protein half-life over a range of mouse tissues, , 2019Protein turnover

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High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients, 2020diaPASEF app note  , , ,

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DOWNLOAD OUR MATERIAL

application note
Towards a Standardized Omics Platform with the 300 samples per day method
APPLICATION NOte 
THE PROTEOMICS DILEMMA – HIGH THROUGHPUT ANALYSIS VERSUS PROTEOME DEPTH
APPLICATION NOTE
GRADIENT OFF-SET FOCUSING HPLC
INSTRUMENT FOR ROBUST AND HIGH
THROUGHPUT CLINICAL PROTEOMICS
BROCHURE
columns and emitters overview

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