| Sensitive and Specific Global Cell Surface N-Glycoproteomics Shows Profound Differences Between Glycosylation Sites and Subcellular Components | DIA | Publication | 2023 | | 30 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Synaptic involvement of the human amygdala in Parkinson’s disease | Clinical proteomics, DIA | Publication | 2023 | | 60 SPD | Bruker timsTOF Pro | Read publication here
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| Comparing extraction method efficiency for high-throughput palaeoproteomic bone species identification | DIA | Publication | 2023 | | 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Ventricular CSF proteomic profiles and predictors of surgical treatment outcome in chronic hydrocephalus | Clinical proteomics, CSF, DIA | Publication | 2023 | | 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| A chemoproteomic platform for selective deubiquitinase inhibitor discovery | DIA | Publication | 2023 | | 30 SPD, 60 SPD | Thermo Orbitrap Fusion Lumos | Read publication here
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| Species-specific metabolic reprogramming in human and mouse microglia during inflammatory pathway induction | DIA | Publication | 2023 | | 30 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Identification of Plasma Biomarkers from Rheumatoid Arthritis Patients Using an Optimized Sequential Window Acquisition of All THeoretical Mass Spectra (SWATH) Proteomics Workflow | DIA | Publication | 2023 | | 60 SPD | Bruker timsTOF Pro | Read publication here
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| Exploration of cell state heterogeneity using single-cell proteomics through sensitivity-tailored data-independent acquisition | DIA, Single Cell Proteomics | Publication | 2023 | | Whisper 20 SPD, Whisper 40 SPD | | Read publication here
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| Exploring the benefits of differential mobility separation and SWATH data independent acquisition for complex proteomic sample analysis | DIA | Poster | 2023 | | 100 SPD, 200 SPD | SCIEX TripleTOF 6600 MS system | Read publication here
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| The potential of plasma HLA peptides beyond neoepitopes | DDA, DIA | Publication | 2023 | | Whisper 40 SPD | Bruker timsTOF Ultra | Read publication here
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| Deep and fast label-free Dynamic Organellar Mapping | DIA, Spatial proteomics | Journal article | 2023 | | 30 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Trans-Omics analysis of post injury thrombo-inflammation identifies endotypes and trajectories in trauma patients | Clinical proteomics, DIA | Publication | 2023 | | | Bruker timsTOF Pro | Read publication here
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| Robust dimethyl-based multiplex-DIA doubles single-cell proteome depth via a reference channel | Automation, DIA, Single Cell Proteomics | Journal article | 2023 | | Whisper 40 SPD | Bruker timsTOF Pro | Read publication here
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| Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae | DIA | Journal article | 2023 | | 30 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Cofilin-Driven Nuclear Deformation Drives Dendritic Cell Migration through the Extracellular Matrix | DIA | Publication | 2023 | | 30 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling | DDA, DIA | Publication | 2023 | | 30 SPD, 60 SPD, Whisper 20 SPD, Whisper 40 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Unleash the power of DIA | DIA | Webinar | 2022 | In this webinar we dive into the exciting research possibilities that comes by using DIA combined with short gradients with Evosep One.
| | | Watch webinar
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| Ultra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation | DIA, Single Cell Proteomics, Technology | Publication | 2020 | In this publication, the Mann group in Münich describes their innovative workflow for single-cell proteomics. They apply a nearly loss-less miniaturized sample preparation workflow to our robust Whisper 40SPD method and combine it with diaPASEF on a modified timsTOF mass spectrometer for a total of 420 FACS sorted single cells.
| Whisper 40 SPD | Bruker timsTOF Pro, Bruker timsTOF SCP | Download publication or learn more on our website
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| DebaryOmics: an integrative –omics study to understand the halophilic behaviour of Debaryomyces hansenii | DIA, Yeast | Publication | 2021 | This publication by the Martinez group at the Technical University of Denmark, presents the first global phosphoproteomics analysis in Debaryomyces hansenii, a non-conventional yeast considered to be a well-suited option for a number of different industrial bioprocesses.
| 30 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Targeted Workflows with Evosep One | DIA, Doping, Targeted workflow | Webinar | 2021 | During this webinar, Michael MacCoss (University of Washington) and Vivian Delcourt (GIE-LCH) share how they use the Evosep One for targeted workflows.
| High Organic Method | | Watch webinar
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| Plasma Proteomics with the Evosep One | DIA | Webinar | 2021 | In this webinar you will learn how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.
| 30 SPD | Bruker timsTOF Pro, Thermo Orbitrap Exploris 480 | Watch webinar
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| AlphaTims: Indexing Trapped Ion Mobility Spectrometry–TOF Data for Fast and Easy Accession and Visualization | DDA, DIA, Technology | Publication | 2021 | This publication by the Mann group, introduces the open-source AlphaTims package with a stand-alone graphical user interface or as part of their AlphaPept ecosystem. With this package, unprocessed data are indexed, accessed, and visualized by a combination of tools.
| 200 SPD, 60 SPD | Bruker timsTOF Pro | Read publication here
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| Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures | Automation, DDA, DIA, T-ALL | Publication | 2021 | In this publication, the Olsen group highlights the potential of proteomics to dissect alterations in cellular signaling and identify druggable pathways in cancer. They identify protein kinase C delta (PKCδ) inhibition as a strategy to overcome resistance to Notch1 inhibition in T-cell acute lymphoblastic leukemia.
| 30 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Unleashing the power of DIA combined with short gradients using Evosep One | DIA, Technology | Webinar | 2021 | This webinar is part of our Evosep webinar series and hosted by Nicolai Bache, Evosep. Florian Meier and Vadim Demichev discuss their use of the Evosep One, using novel DIA methodologies.
| 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Watch webinar
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| Unleashing the true power of DIA/SWATH data acquisition with short gradients – Rapid profiling of pre-clinical models and proteome dynamics | Clinical research, DIA, Technology | Application note | 2019 | Achieving sufficient statistical power for the analysis of a comprehensive set of target proteins requires processing of a large number of samples. With finite resources, researchers face a difficult choice: should they aim at deeper proteomic coverage or higher sample throughput?
| | | Download application note
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| Standardized workflow for precise mid- and high-throughput proteomics of blood biofluids | Automation, Clinical research, DIA | Webinar | 2021 | In this seminar from the Canadian National Proteomics Network (CNPN) meeting 2021, Angela McArdle presents a standardized workflow for precise high-throughput proteomics of blood biofluids. They established optimal conditions for sample preparation and DIA analysis in plasma, then automated and adapted this for depleted plasma and whole blood.
| 60 SPD | Thermo Orbitrap Exploris 480 | Download publication
Watch webinar
Learn more
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| Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers | Clinical research, DIA | Publication | 2021 | In this study, the Theis and Schiller groups at the Helmholtz Zentrum München, investigated the correspondence of cell state changes in diseased organs to peripheral protein signatures in pulmonary fibrosis patient cohorts. From plasma proteome profiling of more than 122 patients, they propose CRTAC1 protein levels in plasma as a novel biomarker.
| 100 SPD, 60 SPD | Thermo Q Exactive HF | Download publication
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| Covid-19 research with Evosep One | Covid-19, DIA, Technology | Webinar | 2021 | This webinar is part of our Evosep webinar series and hosted by Nicolai Bache, Evosep. Angela McArdle and Mathias Trost discuss their use of the Evosep One technology in their development of rapid Covid-19 assays.
| 60 SPD | Thermo Orbitrap Exploris 480 | Watch webinar
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| SPIN – Species by Proteome Investigation | DIA | Publication | 2021 | This publication from the Olsen Group, introduces “Species by Proteome INvestigation” (SPIN), a proteomics workflow capable of querying over 150 mammalian species 200 times a day with the Evosep One. Genetic species determination is an indispensable tool in forensics, archaeology, ecology, and food authentication.
| 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Download publication
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| Spatial-proteomics reveal in-vivo phospho-signaling dynamics at subcellular resolution | DIA, Spatial proteomics | Publication | 2021 | This publication from the Olsen group at the Novo Nordisk Foundation Center for Protein Research, describes a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions.
| 60 SPD | Thermo Orbitrap Exploris 480 | Download publication or read more on our website
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| High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA | DDA, DIA, Technology | Publication | 2020 | High throughput workflows for proteomics
| 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Download publication
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| New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One | DDA, DIA, Technology | Brochure | 2020 | Brochure of Exploris 480 with Evosep data from piece no 1
| 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Download brochure
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| A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-Robust Proteomics | DDA, DIA, Technology | Publication | 2018 | Description of Evosep
| 60 SPD | Thermo Q Exactive HF-X | Download publication
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| High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry | DIA, Technology | Poster | 2020 | BoxCar DIA
| 100 SPD, 30 SPD | Thermo Orbitrap Exploris 480 | Download poster
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| Increasing proteome coverage using cysteine-specific DIA Mass spectrometry – Cys-DIA | DIA | Publication | 2020 | DIA on cysteine-specific peptides
| 60 SPD | Thermo Q Exactive HF-X | Download publication
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| Improving proteome coverage and peptide identification rates in short LC gradients | DDA, DIA, Technology | Video | 2020 | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Watch
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| High-throughput proteomics with Evosep One | DDA, DIA, Technology | Video | 2020 | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Watch
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| Rapid proteome analyses using the Evosep One | DDA, DIA | Video | 2018 | | 60 SPD | Thermo Q Exactive HF | Watch
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| Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine | Clinical research, DIA, DIA (Data Independent Aquisition) | Video | 2019 | View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs.
| 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Watch
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| High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients | DIA, Technology | Application note | 2020 | diaPASEF app note
| 100 SPD, 200 SPD, 300 SPD, 60 SPD | Bruker timsTOF Pro | Download application note
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| Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites | DIA | Publication | 2019 | In this publication, the Olsen Group have combined DIA with short LC gradients to describe how cancer mutations close to tyrosine phosphorylation sites in the EGF receptor rewire signaling pathways by switching protein interactors. The performed 1200 pulldown experiments in just 20 days of LC-MS instrument time.
| 60 SPD | Thermo Q Exactive HF-X | Learn more or download publication
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| Multi-level proteomics reveals host-perturbation strategies of SARS-CoV-2 and SARS-CoV | Clinical research, Covid-19, DIA | Publication | 2020 | This publication describes the molecular functions of viral proteins and their interactions with the host proteome of SARS-CoV-2. The impact of viral infection on the proteome and phosphoproteome were analyzed in a time-resolved manner by DIA. The analysis revealed key pathways perturbed during the infection identifying potential vulnerable points of SARS-CoV-2.
| 30 SPD, 60 SPD | Thermo Q Exactive HF-X | Download publication
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| A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease | Clinical research, DIA | Publication | 2020 | This publication from Matthias Mann groups in Copenhagen and Münich describes a paired liver biopsy and plasma proteomics study, which make use of Boxcar DIA scanning for a large clinical cohort of nearly 600 patients. They have developed a machine learning model based on their biomarker panel, which for the first time outperforms existing tests, …
| 30 SPD | Thermo Q Exactive HF-X | Download or read blog post
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| Increasing Throughput: From Pre-Clinical Models To Protein Complexes | DIA | Video | 2019 | Comparison study with >400 samples, same coverage in shorter time
| 60 SPD | SCIEX | Watch
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| A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients | Automation, DDA, DIA, Technology | Publication | 2020 | Test of Orbitrap Exploris with DIA and TMT, proteome and phospho.
| 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Download
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