The 200 samples per day method

STANDARD METHOD

ULTRA HIGH-THROUGHPUT ANALYSIS

The 200 SPD method is well suited for routine ultra high-throughput applications. The timing and chromatographic performance is maximized to deliver an effective gradient elution of 82% and narrow peaks with FWHM of 1.7 seconds on average.

EXCELLENT SEQUENCING SPEED WITH MOST PEPTIDES PER MINUTE

Good chromatographic quality is essential, and especially to fully employ the high MS/MS scan rates on modern mass spectrometers.

The 200 SPD method provides excellent sequencing speed with most peptides per minute among our standard methods. This translates to a high number of proteins identified per minute.

Maximizing throughput, robustness and analytical depth for shotgun proteomics with the timsTOF Pro

This application note describes the timsTOF PASEF workflow, which enables sequencing speed of more than 120 Hz, high sensitivity and robustness as well as an additional dimension of separation (TIMS) for improved peak capacity. More than 1400 protein groups from 50 ng of HeLa digest were identified with the 200SPD method, all with outstanding robustness and reproducibility.

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SENSITIVE ANALYSIS WITH FAIMS Pro coupled to an orbitrap exploris 480 ms

In this publication the Olsen Group from the Novo Nordisk Foundation Center for Protein Research, Denmark, showcase the sensitivity of the FAIMS Pro device on an Orbitrap Exploris 480 MS by using the 200 SPD method.

They analyzed 5 ng of tryptic peptides equivalent to approximately 30-50 HeLa cells. By using single compensation voltage (CV) of -50, they were able to identify more than 1000 proteins. FAIMS enhanced the reproducibility of DIA quantification, based on the number of proteins quantified with coefficient of variation below 20%.

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USING ARTIFICIAL INTELLIGENCE ON ULTRAFAST LC-MS/MS DIA RUNS FOR BACTERIAL IDENTIFICATION IN URINE

In this seminar, Florence Roux-Dalvai presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs with 200 samples per day.

The work is a collaboration between Québec Research Center, Canada, Thermo Scientific and Evosep Biosystems.

The five standard methods

With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You decide how fast you want to go. 

Standard method

The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.

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Standard method

Excellent sequencing speed with most peptides per minute among our standard methods.

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Standard method

A great “in-between” method with run-to-run reproducibility of just one second.

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Standard method

The most popular method with an excellent balance of throughput and depth.

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Standard method

The longest method with a 44-minute gradient providing the best overall proteome coverage.

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Explore more

Do you want to know how our users are already taking advantage of the 200 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn More
AlphaTims: Indexing trapped ion mobility spectrometry – time of flight data for fast and easy accession and visualization, , 2021This publication by the Mann group, introduces the open-source AlphaTims package with a stand-alone graphical user interface or as part of their AlphaPept ecosystem. With this package, unprocessed data are indexed, accessed, and visualized by a combination of tools.,

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AlphaPept, a modern and open framework for MS-based proteomics, 2021This publication by the Mann group, introduces AlphaPept, a Python-based open-source framework for efficient processing of large high resolution MS data sets. They used the 200 samples per day method on the Evosep One to generate test data for computational functionality.

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Unleashing the power of DIA combined with short gradients using Evosep One, 2021This webinar is part of our Evosep webinar series and hosted by Nicolai Bache, Evosep. Florian Meier and Vadim Demichev discuss their use of the Evosep One, using novel DIA methodologies., ,

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Towards a Standardized Omics Platform with the 200 samples per day method2019This application note describes the 200 samples per day standard method. Get an overview of the method, the chromatographic performance and the results expected from Hela standard injections.

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The proteomics dilemma – High throughput analysis versus proteome depth2019With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis., , , ,

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High sensitivity dia-PASEF proteomics with DIA-NN and FragPipe, 2021The Ralser and Nesvizhskii groups introduce the latest addition to the DIA-NN software with a neural network-based ion mobility module. DIA-NN was originally conceived to maximize the performance of fast proteomics experiments and here they demonstrate the identification of more than 5000 proteins from the 200 SPD method., ,

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Rapid, robust and reproducible host-cell protein analysis with Evosep One, 2021This webinar is part of our Evosep webinar series and hosted by Nicolai Bache, Evosep. Jiao Ma and Anders Giessing discuss their use of the Evosep One to perform rapid, robust and reproducible HCP analysis., ,

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SPIN – Species by Proteome Investigation, 2021This publication from the Olsen Group, introduces “Species by Proteome INvestigation” (SPIN), a proteomics workflow capable of querying over 150 mammalian species 200 times a day with the Evosep One. Genetic species determination is an indispensable tool in forensics, archaeology, ecology, and food authentication., ,

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diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition, 2020A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data completeness with the effective PASEF technology., ,

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High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA, , , , , , , 2020High throughput workflows for proteomics, ,

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New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One, , , 2020Brochure of Exploris 480 with Evosep data from piece no 1, ,

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Online parallel accumulation – serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer, 2018Description of timsTOF

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Improving proteome coverage and peptide identification rates in short LC gradients, , , , , 2020, ,

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High-throughput proteomics with Evosep One, , , , , 2020, ,

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Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine, , 2019View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs.  , ,

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High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients, 2020diaPASEF app note  , , ,

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timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics, 2018Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness., ,

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Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens, , , 2020This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2.,

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A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients, , , , , , , , , 2020Test of Orbitrap Exploris with DIA and TMT, proteome and phospho., ,

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High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition, , 2018High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway., ,

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DOWNLOAD OUR MATERIAL

application note
Towards a Standardized Omics Platform with the 200 samples per day method
APPLICATION NOte 
THE PROTEOMICS DILEMMA – HIGH THROUGHPUT ANALYSIS VERSUS PROTEOME DEPTH
APPLICATION NOTE
GRADIENT OFF-SET FOCUSING HPLC
INSTRUMENT FOR ROBUST AND HIGH
THROUGHPUT CLINICAL PROTEOMICS
BROCHURE
columns and emitters overview

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