The 100 samples per day method
STANDARD METHOD
EXCELLENT RUN-TO-RUN REPRODUCIBILITY OF ONLY ONE SECOND
The 100 SPD method is a great in-between method, which balances proteome coverage and high-throughput with only 2.9 minutes between injections. It provides an excellent solution for routine targeted assays with run-to-run reproducibility of just one second.
FAST CONFIRMATORY ANALYSIS OF rHuEPOs IN PLASMA FOR HORSERACING DOPING CONTROL
In this application note, researchers from LCH, GIE-Laboratories in France describe a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control.
The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing in complex samples such as horse plasma and more generally for targeted proteomics.
The five standard methods
With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You decide how fast you want to go.
Standard method
The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.
Standard method
Excellent sequencing speed with most peptides per minute among our standard methods.
Standard method
A great “in-between” method with run-to-run reproducibility of just one second.
Standard method
The most popular method with an excellent balance of throughput and depth.
Standard method
The longest method with a 44-minute gradient providing the best overall proteome coverage.
Explore more
Do you want to know how our users are already taking advantage of the 300 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.
| Title | Subject | Material | Year | Summary | Institute | Evosep method | MS instrumentation | Learn More |
|---|---|---|---|---|---|---|---|---|
| High-throughput and high-sensitivity biomarker monitoring in body fluid by FAIMS-enhanced fast LC SureQuant™ IS targeted quantitation | Biomarker, FAIMS | Publication | 2022 | 100 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | |||
| Using Targeted Liquid Chromatography-Tandem Mass Spectrometry to Rapidly Detect β-Lactam, Aminoglycoside, and Fluoroquinolone Resistance Mechanisms in Blood Cultures Growing E. coli or K. pneumoniae | Antimicrobial resistance | Publication | 2022 | 100 SPD | Thermo Orbitrap Fusion, Thermo Q Exactive | |||
| ABPP-HT* – Deep meets fast for activity-based profiling of deubiquitylating enzymes using advanced DIA and DDA mass spectrometry methods | DDA, DIA | Publication | 2022 | 100 SPD | timsTOF Pro | |||
| Rapid and Accurate Detection of Aminoglycoside-Modifying Enzymes and 16S rRNA Methyltransferases by Targeted Liquid Chromatography-Tandem Mass | Antimicrobial resistance, Targeted workflow | Publication | 2021 | This publication by the Dekker group describes the development of an accurate and rapid targeted LC-MS/MS assay based on parallel reaction monitoring for detection of the most prevalent aminoglycoside-modifying enzymes and 16S rRNA methyltransferases in Escherichia coli and Klebsiella pneumoniae that confer resistance to aminoglycosides. | 100 SPD | Thermo Q Exactive HF | ||
| A blood atlas of COVID-19 defines hallmarks of disease severity and specificity | Covid-19, DDA, Plasma | Publication | 2021 | This publication is a major collaboration led by the Knight group at University of Oxford describing the hallmarks of Covid-19 severity and specificity. It is an integrative multi-omics analysis, where the Evosep One was used for measuring 340 plasma proteomes providing excellent patient stratification. | 100 SPD | Bruker timsTOF Pro | ||
| Unleashing the power of DIA combined with short gradients using Evosep One | DIA, Technology | Webinar | 2021 | This webinar is part of our Evosep webinar series and hosted by Nicolai Bache, Evosep. Florian Meier and Vadim Demichev discuss their use of the Evosep One, using novel DIA methodologies. | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | ||
| Towards a Standardized Omics Platform with the 100 samples per day method | Technology | Application note | 2019 | This application note describes the 100 samples per day standard method. Get an overview of the method, the chromatographic performance and the results expected from Hela standard injections. | 100 SPD | Thermo Orbitrap Exploris 480 | ||
| Improve and enhance your proteome coverage with the Performance column | Technology | Application note | 2019 | We have developed an additional Performance column for the 60 and 100 samples per day methods (SPD) to address applications with high demands. | 100 SPD, 60 SPD | |||
| The proteomics dilemma – High throughput analysis versus proteome depth | Technology | Application note | 2019 | With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. | 100 SPD, 200 SPD, 30 SPD, 300 SPD, 60 SPD | |||
| High sensitivity dia-PASEF proteomics with DIA-NN and FragPipe | diaPASEF, Technology development | Publication | 2021 | The Ralser and Nesvizhskii groups introduce the latest addition to the DIA-NN software with a neural network-based ion mobility module. DIA-NN was originally conceived to maximize the performance of fast proteomics experiments and here they demonstrate the identification of more than 5000 proteins from the 200 SPD method. | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Download publication or learn more on our website | |
| Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers | Clinical research, DIA, Fibrosis, Plasma | Publication | 2021 | In this study, the Theis and Schiller groups at the Helmholtz Zentrum München, investigated the correspondence of cell state changes in diseased organs to peripheral protein signatures in pulmonary fibrosis patient cohorts. From plasma proteome profiling of more than 122 patients, they propose CRTAC1 protein levels in plasma as a novel biomarker. | 100 SPD, 60 SPD | Thermo Q Exactive HF | ||
| SPIN – Species by Proteome Investigation | Ancient proteomics, DIA | Publication | 2021 | This publication from the Olsen Group, introduces “Species by Proteome INvestigation” (SPIN), a proteomics workflow capable of querying over 150 mammalian species 200 times a day with the Evosep One. Genetic species determination is an indispensable tool in forensics, archaeology, ecology, and food authentication. | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | ||
| ABPP-HT – high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context | IAP-MS, Technology | Publication | 2020 | This publication from University of Oxford led by Adan Pinto-Fernandez, describes a new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics. | 100 SPD | Bruker timsTOF Pro | ||
| diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition | diaPASEF, Technology | Publication | 2020 | A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data completeness with the effective PASEF technology. | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Download publication or learn more on our website
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| High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA | DDA, DIA, FAIMS, Phosphorylation, PTM, Technology, Tissue, TMT | Publication | 2020 | High throughput workflows for proteomics | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | ||
| Optimizing data processing parameters for samples acquired on instruments using Advanced Peak Determination | DDA, Technology | Poster | 2018 | Improving ID rates with APD on Q Exactive HF-X | 100 SPD | Thermo Q Exactive HF-X | ||
| New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One | DDA, DIA, FAIMS, Technology | Brochure | 2020 | Brochure of Exploris 480 with Evosep data from piece no 1 | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | ||
| High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry | DIA, Plasma, Technology | Poster | 2020 | BoxCar DIA | 100 SPD, 30 SPD | Thermo Orbitrap Exploris 480 | ||
| Improving proteome coverage and peptide identification rates in short LC gradients | DDA, DIA, FAIMS, Phosphorylation, PTM, Technology | Video | 2020 | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | |||
| High-throughput proteomics with Evosep One | DDA, DIA, FAIMS, Phosphoproteomics, PTM, Technology | Video | 2020 | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | |||
| Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine | Bacteria, Clinical research, DIA | Video | 2019 | View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs. | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | ||
| High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients | DIA, Technology | Application note | 2020 | diaPASEF app note | 100 SPD, 200 SPD, 300 SPD, 60 SPD | Bruker timsTOF Pro | ||
| MaxQuant software for ion moiblity enhanced shotgun proteomics | DDA, Plasma, Technology | Publication | 2020 | MaxQuant for timsTOF analysis | 100 SPD | Bruker timsTOF Pro | ||
| Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min | Clinical research, DDA, Plasma | Application note | 2019 | The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients). | 100 SPD | Bruker timsTOF Pro | ||
| Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform | Clinical research, DDA, Lung, Plasma, Serum | Application note | 2019 | In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard. | 100 SPD, 30 SPD, 60 SPD | Thermo Q Exactive HF-X | ||
| timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics | DDA, Technology | Application note | 2018 | Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness. | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | ||
| Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens | Clinical research, Covid-19, DDA, Targeted workflow | Publication | 2020 | This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2. | 100 SPD, 200 SPD | Thermo Orbitrap Exploris 480 | ||
| A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients | Automation, DDA, DIA, FAIMS, Offline fractionation, Phosphoproteomics, PTM, Technology, Tissue, TMT | Publication | 2020 | Test of Orbitrap Exploris with DIA and TMT, proteome and phospho. | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | ||
| Accurate Detection Of The Four Most Prevalent Carbapenemases In E. Coli And K. Pneumoniae By High-Resolution Mass Spectrometry | Bacteria, Targeted workflow | Publication | 2019 | “Proof of concept” study showing the potential of targeted high resolution MS in detecting different resistance mechanisms. Accurate detection of KPC, OXA-48-like, NDM and VIM carbapenemases. | 100 SPD | Thermo Q Exactive HF | ||
| High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition | Phosphorylation, PTM, Targeted workflow | Poster | 2018 | High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway. | 100 SPD, 200 SPD, 60 SPD | Thermo Q Exactive HF-X | ||
| Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control | Plasma, rHuEPO, Targeted workflow | Application note | 2019 | This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing. | 100 SPD | Thermo Q Exactive HF |
DOWNLOAD OUR MATERIAL
application note
Towards a Standardized Omics Platform with the 100 samples per day method
APPLICATION NOte
THE PROTEOMICS DILEMMA – HIGH THROUGHPUT ANALYSIS VERSUS PROTEOME DEPTH
APPLICATION NOTE
GRADIENT OFF-SET FOCUSING HPLC INSTRUMENT FOR ROBUST AND HIGH
THROUGHPUT CLINICAL PROTEOMICS
application note
FAST CONFIRMATORY ANALYSIS OF RHUEPOS IN PLASMA FOR HORSERACING DOPING CONTROL
application note
Improve and enhance your proteome coverage with the Performance column
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