The 100 samples per day method
STANDARD METHOD
EXCELLENT RUN-TO-RUN REPRODUCIBILITY OF ONLY ONE SECOND
The 100 SPD method is a great in-between method, which balances proteome coverage and high-throughput with only 2.9 minutes between injections. It provides an excellent solution for routine targeted assays with run-to-run reproducibility of just one second.
FAST CONFIRMATORY ANALYSIS OF rHuEPOs IN PLASMA FOR HORSERACING DOPING CONTROL
In this application note, researchers from LCH, GIE-Laboratories in France describe a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control.
The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing in complex samples such as horse plasma and more generally for targeted proteomics.
The five standard methods
With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You device how fast you want to go.
Standard method
The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.
Standard method
Excellent sequencing speed with most peptides per minute among our standard methods.
Standard method
A great “in-between” method with run-to-run reproducibility of just one second.
Standard method
The longest method with a 44-minute gradient providing the best overall proteome coverage.
Explore more
Do you want to know how our users are already taking advantage of the 300 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.
| Title | Subject | Material | Year | Summary | Institute | Evosep method | MS instrumentation | Learn More |
|---|---|---|---|---|---|---|---|---|
| ABPP-HT – high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context | IAP-MS, Technology | Publication | 2020 | This publication from University of Oxford led by Adan Pinto-Fernandez, describes a new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics. | University of Oxford, United Kingdom. | 100 SPD | Bruker timsTOF Pro | |
| diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition | diaPASEF, Technology | Publication | 2020 | A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data completeness with the effective PASEF technology. | Max Planck Institute of Biochemistry, Martinsried, Germany. Bruker Daltonik, Germany. ETH Zürich, Switzerland. University of Belfast, United Kingdom. University of Toronto, Canada. | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Download publication or learn more on our website
|
| High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA | DDA, DIA, FAIMS, Phosphorylation, PTM, Technology, Tissue, TMT | Publication | 2020 | High throughput workflows for proteomics | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | |
| Optimizing data processing parameters for samples acquired on instruments using Advanced Peak Determination | DDA, Technology | Poster | 2018 | Improving ID rates with APD on Q Exactive HF-X | Thermo Fisher Scientific, United States | 100 SPD | Thermo Q Exactive HF-X | |
| New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One | DDA, DIA, FAIMS, Technology | Brochure | 2020 | Brochure of Exploris 480 with Evosep data from piece no 1 | Thermo Fisher Scientific, United States | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | |
| High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry | DIA, Plasma, Technology | Poster | 2020 | BoxCar DIA | Max Planck Institute of Biochemistry, Martinsried, Germany | 100 SPD, 30 SPD | Thermo Orbitrap Exploris 480 | |
| Improving proteome coverage and peptide identification rates in short LC gradients | DDA, DIA, FAIMS, Phosphorylation, PTM, Technology | Video | 2020 | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | ||
| High-throughput proteomics with Evosep One | DDA, DIA, FAIMS, Phosphoproteomics, PTM, Technology | Video | 2020 | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | ||
| Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine | Bacteria, Clinical research, DIA | Video | 2019 | View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs. | CHU Québec Université Laval Research Center, Canada | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | |
| High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients | DIA, Technology | Application note | 2020 | diaPASEF app note | Bruker Daltonics, Germany | 100 SPD, 200 SPD, 300 SPD, 60 SPD | Bruker timsTOF Pro | |
| MaxQuant software for ion moiblity enhanced shotgun proteomics | DDA, Plasma, Technology | Publication | 2020 | MaxQuant for timsTOF analysis | Bruker Daltonics, Germany | 100 SPD | Bruker timsTOF Pro | |
| Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min | Clinical research, DDA, Plasma | Application note | 2019 | The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients). | Bruker Daltonics, Germany | 100 SPD | Bruker timsTOF Pro | |
| Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform | Clinical research, DDA, Lung, Plasma, Serum | Application note | 2019 | In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard. | Thermo Fisher Scientific, United States | 100 SPD, 30 SPD, 60 SPD | Thermo Q Exactive HF-X | |
| timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics | DDA, Technology | Application note | 2018 | Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness. | Bruker Daltonics, Germany | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | |
| Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens | Clinical research, Covid-19, DDA, Targeted workflow | Publication | 2020 | This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2. | Mayo Clinic Rochester, United States | 100 SPD, 200 SPD | Thermo Orbitrap Exploris 480 | |
| A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients | Automation, DDA, DIA, FAIMS, Offline fractionation, Phosphoproteomics, PTM, Technology, Tissue, TMT | Publication | 2020 | Test of Orbitrap Exploris with DIA and TMT, proteome and phospho. | Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | |
| Accurate Detection Of The Four Most Prevalent Carbapenemases In E. Coli And K. Pneumoniae By High-Resolution Mass Spectrometry | Bacteria, Targeted workflow | Publication | 2019 | “Proof of concept” study showing the potential of targeted high resolution MS in detecting different resistance mechanisms. Accurate detection of KPC, OXA-48-like, NDM and VIM carbapenemases. | Erasmus MC, The Netherlands | 100 SPD | Thermo Q Exactive HF | |
| High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition | Phosphorylation, PTM, Targeted workflow | Poster | 2018 | High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway. | Thermo Fisher Scientific, United States | 100 SPD, 200 SPD, 60 SPD | Thermo Q Exactive HF-X | |
| Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control | Plasma, rHuEPO, Targeted workflow | Application note | 2019 | This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing. | Le Laboratoire des Courses Hippiques (LCH), France | 100 SPD | Thermo Q Exactive HF |
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