The 100 samples per day method

STANDARD METHOD

EXCELLENT RUN-TO-RUN REPRODUCIBILITY OF ONLY ONE SECOND

The 100 SPD method is a great in-between method, which balances proteome coverage and high-throughput with only 2.9 minutes between injections. It provides an excellent solution for routine targeted assays with run-to-run reproducibility of just one second.

FAST CONFIRMATORY ANALYSIS OF rHuEPOs IN PLASMA FOR HORSERACING DOPING CONTROL

In this application note, researchers from LCH, GIE-Laboratories in France describe a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control.

The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing in complex samples such as horse plasma and more generally for targeted proteomics.

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The five standard methods

With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You device how fast you want to go. 

Standard method

The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.

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Standard method

Excellent sequencing speed with most peptides per minute among our standard methods.

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Standard method

A great “in-between” method with run-to-run reproducibility of just one second.

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Standard method

The most popular method with an excellent balance of throughput and depth.

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Standard method

The longest method with a 44-minute gradient providing the best overall proteome coverage.

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Explore more

Do you want to know how our users are already taking advantage of the 300 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn More
Towards a Standardized Omics Platform with the 100 samples per day method2019This method has an 11.5 minute gradient and a cycle time of 14.4 minutes.

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Improve and enhance your proteome coverage with the Performance column2019We have developed an additional Performance column for the 60 and 100 samples per day methods (SPD) to address applications with high demands.,

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The proteomics dilemma – High throughput analysis versus proteome depth2019With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis., , , ,

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High sensitivity dia-PASEF proteomics with DIA-NN and FragPipe, 2021The Ralser and Nesvizhskii groups introduce the latest addition to the DIA-NN software with a neural network-based ion mobility module. DIA-NN was originally conceived to maximize the performance of fast proteomics experiments and here they demonstrate the identification of more than 5000 proteins from the 200 SPD method., ,

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Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers, , , 2021In this study, the Theis and Schiller groups at the Helmholtz Zentrum München, investigated the correspondence of cell state changes in diseased organs to peripheral protein signatures in pulmonary fibrosis patient cohorts. From plasma proteome profiling of more than 122 patients, they propose CRTAC1 protein levels in plasma as a novel biomarker.,

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SPIN – Species by Proteome Investigation, 2021This publication from the Olsen Group, introduces “Species by Proteome INvestigation” (SPIN), a proteomics workflow capable of querying over 150 mammalian species 200 times a day with the Evosep One. Genetic species determination is an indispensable tool in forensics, archaeology, ecology, and food authentication., ,

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ABPP-HT – high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context, 2020This publication from University of Oxford led by Adan Pinto-Fernandez, describes a new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics.

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diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition, 2020A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data completeness with the effective PASEF technology., ,

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High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA, , , , , , , 2020High throughput workflows for proteomics, ,

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Optimizing data processing parameters for samples acquired on instruments using Advanced Peak Determination, 2018Improving ID rates with APD on Q Exactive HF-X

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New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One, , , 2020Brochure of Exploris 480 with Evosep data from piece no 1, ,

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High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry, , 2020BoxCar DIA,

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Improving proteome coverage and peptide identification rates in short LC gradients, , , , , 2020, ,

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High-throughput proteomics with Evosep One, , , , , 2020, ,

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Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine, , 2019View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs.  , ,

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High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients, 2020diaPASEF app note  , , ,

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MaxQuant software for ion moiblity enhanced shotgun proteomics, , 2020MaxQuant for timsTOF analysis

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Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min, , 2019The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients).

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Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform, , , , 2019In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard., ,

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timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics, 2018Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness., ,

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Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens, , , 2020This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2.,

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A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients, , , , , , , , , 2020Test of Orbitrap Exploris with DIA and TMT, proteome and phospho., ,

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Accurate Detection Of The Four Most Prevalent Carbapenemases In E. Coli And K. Pneumoniae By High-Resolution Mass Spectrometry, 2019“Proof of concept” study showing the potential of targeted high resolution MS in detecting different resistance mechanisms. Accurate detection of KPC, OXA-48-like, NDM and VIM carbapenemases.

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High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition, , 2018High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway., ,

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Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control, , 2019This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing.

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DOWNLOAD OUR MATERIAL

application note
Towards a Standardized Omics Platform with the 100 samples per day method
APPLICATION NOte 
THE PROTEOMICS DILEMMA – HIGH THROUGHPUT ANALYSIS VERSUS PROTEOME DEPTH
APPLICATION NOTE
GRADIENT OFF-SET FOCUSING HPLC INSTRUMENT FOR ROBUST AND HIGH
THROUGHPUT CLINICAL PROTEOMICS
application note
FAST CONFIRMATORY ANALYSIS OF RHUEPOS IN PLASMA FOR HORSERACING DOPING CONTROL
application note
Improve and enhance your proteome coverage with the Performance column

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