| Loss of Lipooligosaccharide Synthesis in Acinetobacter baumannii Produces Changes in Outer Membrane Vesicle Protein Content | | Publication | 2024 | | | 100 SPD | Bruker timsTOF fleX | Read publication here
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| A modular DNA origami nanocompartment for engineering a cell-free, protein unfolding and degradation pathway | | Publication | 2024 | | | 100 SPD, 60 SPD | | Read publication here
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| Towards routine proteome profiling of FFPE tissue: Insights from a 1,200 case pan-cancer study | | Publication | 2024 | | | 100 SPD, 15 SPD, 30 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Evolocumab as an immunomodulator in glioma: A window of opportunity trial evaluating PCSK9 inhibition to enhance surface MHC-I on tumor | | Publication | 2024 | | | 100 SPD, 60 SPD | Thermo Orbitrap Astral | Read publication here
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| Single cell proteomics by mass spectrometry reveals deep epigenetic insight into the actions of an orphan histone deacetylase inhibitor | | Publication | 2024 | | | 100 SPD, 200 SPD, 30 SPD, 500 SPD, 60 SPD | Bruker timsTOF SCP | Read publication here
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| A complete and automated end-to-end sample preparation strategy for high-throughput and standardized proteomics with high sensitivity | | Poster | 2024 | | | 100 SPD, 500 SPD, Whisper Zoom 40 SPD, Whisper Zoom 80 SPD | Bruker timsTOF HT | Read poster here
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| Cost-efficient and scalable end-to-end workflow on the Opentrons OT-2 for neat plasma utilizing Evotip Pure | | Application note | 2024 | | | 100 SPD, 200 SPD | Bruker timsTOF HT | Read application note here
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| Efficient proteomics with an automated sample preparation strategy leveraged by Evotip Pure and the AssayMAP Bravo | | Application note | 2024 | | | 100 SPD, 500 SPD, Whisper Zoom 40 SPD, Whisper Zoom 80 SPD | Bruker timsTOF HT | Read application note here
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| Unlocking the plasma proteome | | Application note | 2024 | | | 100 SPD | Thermo Orbitrap Astral | Read application note here
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| High-throughput mass spectrometry maps the sepsis plasma proteome and differences in patient response | | Publication | 2024 | | | 100 SPD | timsTOF Pro | Read publication here
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| Fully automated workflow for integrated sample digestion and Evotip loading enabling high-throughput clinical proteomics | | Publication | 2024 | | | 100 SPD, 60 SPD | Thermo Orbitrap Astral, Thermo Orbitrap Exploris 480 | Read publication here
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| Cost-efficient and scalable end-to-end workflow on the Opentrons OT-2 for neat plasma utilizing Evotip Pure | Automation, Plasma | Application note | 2024 | Plasma proteomics stands at the forefront of biomedical research as it offers invaluable insights into disease progression and holds immense potential for minimally invasive diagnostics. With recent advancements toward faster LC-MS analysis, the demand for robust, high-throughput, and cost-efficient sample preparation has increased. To address this, we present a fully automated plasma sample preparation workflow … | | 100 SPD, 200 SPD | Bruker timsTOF HT, SCIEX ZenoTOF 7600 | Download application note here
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| Efficient proteomics with an automated sample preparation strategy leveraged by Evotip Pure and the AssayMAP Bravo | Automation | Application note | 2024 | Proteomics is undergoing a significant shift and rapidly evolving towards more efficient and user-friendly technologies that can handle higher quantities of samples. The Evosep One, combinedwith improvements in high-end mass spectrometry, has played a vital role in transforming this field, making it possible to analyze hundreds of proteomes daily. Consequently, the bottleneck in high-throughput proteomics … | | 100 SPD, 500 SPD, Whisper Zoom 40 SPD, Whisper Zoom 80 SPD | Bruker timsTOF HT | Download application note here
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| Unlocking the plasma proteome with Evotip Pure based, standardized and scalable workflows | Automation, Plasma | Application note | 2024 | Plasma stands out as one of the most readily accessible biofluids, with an estimated pres- ence of over 10,000 distinct proteins. A significant portion of these are released from organs and tissues. The ability to measure such proteins in a robust manner holds tremendous potential for advancing next-generation multiplexed biomarker discovery. However, methods must be … | | 100 SPD | Thermo Orbitrap Astral | Download application note here
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| Increasing sustainability in palaeoproteomics by optimizing digestion times for large-scale archaeological bone analyses | | Publication | 2024 | | | 100 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Probe-dependent Proximity Profiling (ProPPr) Uncovers Similarities and Differences in Phospho-Tau-Associated Proteomes Between Tauopathies | | Publication | 2024 | | | 100 SPD | timsTOF Pro 2 | Read publication here
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| Proteomic Analysis Reveals Trilaciclib-Induced Senescence | | Publication | 2024 | | | 100 SPD | Bruker timsTOF HT | Read publication here
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| Direct Relationship between Protein Expression and Progeny Yield of Herpes Simplex Virus 1 Unveils a Rate-limiting Step for Virus Production | | Publication | 2024 | | | 100 SPD | Thermo Q Exactive HF-X | Read publication here
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| Neat plasma proteomics: getting the best out of the worst | | Publication | 2024 | | | 100 SPD, 60 SPD, Whisper 40 SPD | timsTOF Pro | Read publication here
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| diaPASEF Enables High-throughput Proteomic Analysis of Host Cell Proteins for Biopharmaceutical Process Development | | Publication | 2024 | | | 100 SPD, 15 SPD, 30 SPD | timsTOF Pro 2 | Read publication here
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| PU.1 Eviction at Lymphocyte-Specific Chromatin Domains Mediates Glucocorticoid Response in Acute Lymphoblastic Leukemia | | Publication | 2024 | | | 100 SPD | Bruker timsTOF HT | Read publication here
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| Fragment ion intensity prediction improves the identification rate of non-tryptic peptides in timsTOF | | Publication | 2024 | | | 100 SPD | timsTOF Pro | Read publication here
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| timsTOF HT Improves Protein Identification and Quantitative Reproducibility for Deep Unbiased Plasma Protein Biomarker Discovery | | Publication | 2024 | | | 100 SPD, 30 SPD, 60 SPD | Bruker timsTOF HT, timsTOF Pro 2 | Read publication here
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| Full Mass Range ΦSDM Orbitrap Mass Spectrometry for DIA Proteome Analysis | | Publication | 2024 | | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Single cell proteomics by mass spectrometry reveals deep epigenetic insight and new targets of a class specific histone deacetylase inhibitor | | Publication | 2024 | | | 100 SPD, 200 SPD, 30 SPD, 300 SPD, 500 SPD, 60 SPD | Bruker timsTOF SCP | Read publication here
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| Fully automated workflow for integrated sample digestion and Evotip loading enabling high-throughput clinical proteomics | | Publication | 2023 | | | 100 SPD, 30 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| Production in Nicotiana benthamiana of a thermotolerant glucose oxidase that shows enzybiotic activity against Escherichia coli and Staphylococcus aureus | | Publication | 2023 | | | 100 SPD | Bruker timsTOF fleX | Read publication here
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| Exploring the benefits of differential mobility separation and SWATH data independent acquisition for complex proteomic sample analysis | DIA | Poster | 2023 | | | 100 SPD, 200 SPD | SCIEX TripleTOF 6600 MS system | Read publication here
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| Comparative proteomics analysis in different stages of urothelial bladder cancer for identification of potential biomarkers: highlighted role for antioxidant activity | Cancer, Clinical proteomics | Journal article | 2023 | | | 100 SPD | timsTOF Pro 2 | Read publication here
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| A complete and automated end-to-end sample preparation strategy for high-throughput and standardized proteomics with high sensitivity | | Poster | 2023 | | | 100 SPD, 30 SPD, Whisper 40 SPD | | Read app note here
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| Rapid and robust PTM peptide mapping for biologics with the Evosep One and the ZenoTOF 7600 | | Poster | 2023 | | | 100 SPD, 200 SPD, 300 SPD, 60 SPD | SCIEX ZenoTOF 7600 | Read app note here
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| Vacuum Insulated Probe Heated Electrospray Ionization Source Enhances Microflow Rate Chromatography Signals in the Bruker timsTOF Mass Spectrometer | | Publication | 2023 | | | 100 SPD, 15 SPD, 200 SPD, 30 SPD, 60 SPD | Bruker timsTOF | Read publication here
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| Evotip Pure simplifies workflows with excellent reproducibility, storage and recovery | | Application note | 2023 | The Evotip plays a vital role in the workflow of the Evosep One. It seamlessly integrates front-end sample preparation with liquid chromatography, facilitating efficient mass spectrometry analysis. Evotip Pure can be utilized for sensitive targeted assays, here illustrated in combination with the Agilent 6495C | | 100 SPD, 60 SPD, Whisper 40 SPD | | Download app note
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| Towards a Standardized Omics Platform with the 100 samples per day method | Technology | Application note | 2023 | This application note describes the 100 samples per day standard method. Get an overview of the method, the chromatographic performance and the results expected from Hela standard injections. | | 100 SPD | Thermo Orbitrap Exploris 480 | Download brochure
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| Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth | Fractionation, Technology | Application note | 2023 | The Evosep One offers a diverse range of applications, utilizing its six standard methods. It caters to various use cases, spanning from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. | | 100 SPD, 200 SPD, 30 SPD, 300 SPD, 60 SPD | | Download application note
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| End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-2 | Automation | Application note | 2023 | The Evosep One provides a standardized and user-friendly platform that enables routine analysis of hundreds of proteomes per instru- ment per day, which has shifted the bottleneck for realizing high-throughput proteomics towards sample preparation. To address this, we have developed a fully automated Evotip loading protocol, based on a unique layered sandwich approach, which is … | | 100 SPD, Whisper 40 SPD | timsTOF Pro 2 | Download application note here
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| Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo | Automation | Application note | 2023 | Automation and robotics have revolutionized the field of proteomics, offering solutions to the growing need for streamlined workflows and efficient sample processing. In this context, automated loading of Evotips has emerged as a pivotal advancement in sample preparation workflows. The Evotip can now be loaded using an innovative and fully automated strategy with the Agilent … | | 100 SPD, Whisper 40 SPD | SCIEX ZenoTOF 7600, timsTOF Pro 2 | Download application note here
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| Flexibility, speed, and throughput for high proteome coverage using Zeno SWATH data-independent acquisition (DIA) coupled with the Evosep One system | | Application note | 2023 | | | 100 SPD, 200 SPD, 30 SPD, 300 SPD, 500 SPD, 60 SPD | SCIEX ZenoTOF 7600, timsTOF Pro 2 | Read publication here
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| Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer | | Publication | 2023 | | | 100 SPD, 200 SPD, 30 SPD, 60 SPD, Extended method | Bruker timsTOF Pro | Read publication here
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| Chromatographic separation of peptides and proteins for characterization of proteomes | | Journal article | 2022 | | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Read publication here
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| A complete, automated Opentrons OT-2 loading protocol for simplified sample loading of Evotip Pure | | Application note | 2022 | | | 100 SPD, 500 SPD, Whisper 40 SPD | Bruker timsTOF Pro | Read application note here
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| Rapid and robust PTM peptide mapping for biologics with the Evosep One and the SCIEX ZenoTOF 7600 | | Application note | 2022 | | | 100 SPD, 300 SPD, 60 SPD | SCIEX ZenoTOF 7600 | Read application note here
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| Synchro-PASEF allows precursor-specific fragment ion extraction and interference removal in data-independent acquisition | | Publication | 2022 | | | 100 SPD, 60 SPD | Bruker timsTOF Pro | Read publication here
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| Extended detection of anabolics misuse in high-throughput doping controls with the Evosep One | | Application note | 2022 | | | 100 SPD | | Read application note
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| High-throughput mass spectrometry maps the sepsis plasma proteome and differences in response | | Publication | 2022 | | | 100 SPD | Bruker timsTOF Pro | Read publication here
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| High-throughput quantitative proteomics using Zeno SWATH data-independent analysis (DIA) and the Evosep One system | | Application note | 2022 | | | 100 SPD, 200 SPD, 30 SPD, 60 SPD | SCIEX ZenoTOF 7600 | Read publication here
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| Rapid and in-depth coverage of the (phospho-)proteome with deep libraries and optimal window design for dia-PASEF | | Publication | 2022 | | | 100 SPD, 30 SPD, 60 SPD | Bruker timsTOF Pro | Read publication here
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| Using Targeted Liquid Chromatography-Tandem Mass Spectrometry to Rapidly Detect β-Lactam, Aminoglycoside, and Fluoroquinolone Resistance Mechanisms in Blood Cultures Growing E. coli or K. pneumoniae | | Publication | 2022 | | | 100 SPD | Thermo Orbitrap Fusion, Thermo Q Exactive | Read publication here
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| ABPP-HT* – Deep meets fast for activity-based profiling of deubiquitylating enzymes using advanced DIA and DDA mass spectrometry methods | | Publication | 2022 | | | 100 SPD | Bruker timsTOF Pro | Read publication here
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| Rapid and Accurate Detection of Aminoglycoside-Modifying Enzymes and 16S rRNA Methyltransferases by Targeted Liquid Chromatography-Tandem Mass | Antimicrobial resistance, Targeted workflow | Publication | 2021 | This publication by the Dekker group describes the development of an accurate and rapid targeted LC-MS/MS assay based on parallel reaction monitoring for detection of the most prevalent aminoglycoside-modifying enzymes and 16S rRNA methyltransferases in Escherichia coli and Klebsiella pneumoniae that confer resistance to aminoglycosides. | | 100 SPD | Thermo Q Exactive HF | Read publication here
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| A blood atlas of COVID-19 defines hallmarks of disease severity and specificity | Covid-19, DDA, Plasma | Publication | 2021 | This publication is a major collaboration led by the Knight group at University of Oxford describing the hallmarks of Covid-19 severity and specificity. It is an integrative multi-omics analysis, where the Evosep One was used for measuring 340 plasma proteomes providing excellent patient stratification. | | 100 SPD | Bruker timsTOF Pro | Read publication here
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| Unleashing the power of DIA combined with short gradients using Evosep One | DIA, Technology | Webinar | 2021 | This webinar is part of our Evosep webinar series and hosted by Nicolai Bache, Evosep. Florian Meier and Vadim Demichev discuss their use of the Evosep One, using novel DIA methodologies. | | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Watch webinar
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| Improve and enhance your proteome coverage with the Performance column | Technology | Application note | 2019 | We have developed an additional Performance column for the 60 and 100 samples per day methods (SPD) to address applications with high demands. | | 100 SPD, 60 SPD | | Download application note
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| High sensitivity dia-PASEF proteomics with DIA-NN and FragPipe | diaPASEF, Technology development | Publication | 2021 | The Ralser and Nesvizhskii groups introduce the latest addition to the DIA-NN software with a neural network-based ion mobility module. DIA-NN was originally conceived to maximize the performance of fast proteomics experiments and here they demonstrate the identification of more than 5000 proteins from the 200 SPD method. | | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Download publication or learn more on our website
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| Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers | Clinical research, DIA, Fibrosis, Plasma | Publication | 2021 | In this study, the Theis and Schiller groups at the Helmholtz Zentrum München, investigated the correspondence of cell state changes in diseased organs to peripheral protein signatures in pulmonary fibrosis patient cohorts. From plasma proteome profiling of more than 122 patients, they propose CRTAC1 protein levels in plasma as a novel biomarker. | | 100 SPD, 60 SPD | Thermo Q Exactive HF | Download publication
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| SPIN – Species by Proteome Investigation | Ancient proteomics, DIA | Publication | 2021 | This publication from the Olsen Group, introduces “Species by Proteome INvestigation” (SPIN), a proteomics workflow capable of querying over 150 mammalian species 200 times a day with the Evosep One. Genetic species determination is an indispensable tool in forensics, archaeology, ecology, and food authentication. | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Download publication
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| ABPP-HT – high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context | IAP-MS, Technology | Publication | 2020 | This publication from University of Oxford led by Adan Pinto-Fernandez, describes a new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics. | | 100 SPD | Bruker timsTOF Pro | Download publication
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| diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition | diaPASEF, Technology | Publication | 2020 | A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data completeness with the effective PASEF technology. | | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Download publication or learn more on our website
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| High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA | DDA, DIA, FAIMS, Phosphorylation, PTM, Technology, Tissue, TMT | Publication | 2020 | High throughput workflows for proteomics | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Download publication
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| Optimizing data processing parameters for samples acquired on instruments using Advanced Peak Determination | DDA, Technology | Poster | 2018 | Improving ID rates with APD on Q Exactive HF-X | | 100 SPD | Thermo Q Exactive HF-X | Download poster
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| New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One | DDA, DIA, FAIMS, Technology | Brochure | 2020 | Brochure of Exploris 480 with Evosep data from piece no 1 | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Download brochure
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| High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry | DIA, Plasma, Technology | Poster | 2020 | BoxCar DIA | | 100 SPD, 30 SPD | Thermo Orbitrap Exploris 480 | Download poster
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| Improving proteome coverage and peptide identification rates in short LC gradients | DDA, DIA, FAIMS, Phosphorylation, PTM, Technology | Video | 2020 | | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Watch
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| High-throughput proteomics with Evosep One | DDA, DIA, FAIMS, Phosphoproteomics, PTM, Technology | Video | 2020 | | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Watch
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| Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine | Bacteria, Clinical research, DIA | Video | 2019 | View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs. | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Watch
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| High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients | DIA, Technology | Application note | 2020 | diaPASEF app note | | 100 SPD, 200 SPD, 300 SPD, 60 SPD | Bruker timsTOF Pro | Download application note
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| MaxQuant software for ion moiblity enhanced shotgun proteomics | DDA, Plasma, Technology | Publication | 2020 | MaxQuant for timsTOF analysis | | 100 SPD | Bruker timsTOF Pro | Download publication
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| Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min | Clinical research, DDA, Plasma | Application note | 2019 | The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients). | | 100 SPD | Bruker timsTOF Pro | Download publication
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| Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform | Clinical research, DDA, Lung, Plasma, Serum | Application note | 2019 | In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard. | | 100 SPD, 30 SPD, 60 SPD | Thermo Q Exactive HF-X | Learn more or download application note
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| timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics | DDA, Technology | Application note | 2018 | Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness. | | 100 SPD, 200 SPD, 60 SPD | Bruker timsTOF Pro | Download publication or download poster
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| Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens | Clinical research, Covid-19, DDA, Targeted workflow | Publication | 2020 | This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2. | | 100 SPD, 200 SPD | Thermo Orbitrap Exploris 480 | Download publication
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| A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients | Automation, DDA, DIA, FAIMS, Fractionation, Phosphoproteomics, PTM, Technology, Tissue, TMT | Publication | 2020 | Test of Orbitrap Exploris with DIA and TMT, proteome and phospho. | | 100 SPD, 200 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | Download
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| Accurate Detection Of The Four Most Prevalent Carbapenemases In E. Coli And K. Pneumoniae By High-Resolution Mass Spectrometry | Bacteria, Targeted workflow | Publication | 2019 | “Proof of concept” study showing the potential of targeted high resolution MS in detecting different resistance mechanisms. Accurate detection of KPC, OXA-48-like, NDM and VIM carbapenemases. | | 100 SPD | Thermo Q Exactive HF | Download
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| High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition | Phosphorylation, PTM, Targeted workflow | Poster | 2018 | High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway. | | 100 SPD, 200 SPD, 60 SPD | Thermo Q Exactive HF-X | Download
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| Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control | Plasma, rHuEPO, Targeted workflow | Application note | 2019 | This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing. | | 100 SPD | Thermo Q Exactive HF | Download
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