The 100 samples per day method

STANDARD METHOD

EXCELLENT RUN-TO-RUN REPRODUCIBILITY OF ONLY ONE SECOND

The 100 SPD method is a great in-between method, which balances proteome coverage and high-throughput with only 2.9 minutes between injections. It provides an excellent solution for routine targeted assays with run-to-run reproducibility of just one second.

FAST CONFIRMATORY ANALYSIS OF rHuEPOs IN PLASMA FOR HORSERACING DOPING CONTROL

In this application note, researchers from LCH, GIE-Laboratories in France describe a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control.

The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing in complex samples such as horse plasma and more generally for targeted proteomics.

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The six standard methods

With the six standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You decide how fast you want to go. 

Standard method

The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.

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Standard method

Very high peak capacity per minute for high-throughput analysis.

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Standard method

Excellent sequencing speed with most peptides per minute among our standard methods.

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Standard method

A great “in-between” method with run-to-run reproducibility of just one second.

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Standard method

The most popular method with an excellent balance of throughput and depth.

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Standard method

The longest method with a 44-minute gradient providing the best overall proteome coverage.

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Explore more

Do you want to know how our users are already taking advantage of the 300 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn More
High-throughput mass spectrometry maps the sepsis plasma proteome and differences in patient response2024

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Fully automated workflow for integrated sample digestion and Evotip loading enabling high-throughput clinical proteomics2024, ,

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Cost-efficient and scalable end-to-end workflow on the Opentrons OT-2 for neat plasma utilizing Evotip Pure, 2024Plasma proteomics stands at the forefront of biomedical research as it offers invaluable insights into disease progression and holds immense potential for minimally invasive diagnostics. With recent advancements toward faster LC-MS analysis, the demand for robust, high-throughput, and cost-efficient sample preparation has increased. To address this, we present a fully automated plasma sample preparation workflow …, ,

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Efficient proteomics with an automated sample preparation strategy leveraged by Evotip Pure and the AssayMAP Bravo2024Proteomics is undergoing a significant shift and rapidly evolving towards more efficient and user-friendly technologies that can handle higher quantities of samples. The Evosep One, combinedwith improvements in high-end mass spectrometry, has played a vital role in transforming this field, making it possible to analyze hundreds of proteomes daily. Consequently, the bottleneck in high-throughput proteomics …, , ,

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Unlocking the plasma proteome with Evotip Pure based, standardized and scalable workflows, 2024Plasma stands out as one of the most readily accessible biofluids, with an estimated pres- ence of over 10,000 distinct proteins. A significant portion of these are released from organs and tissues. The ability to measure such proteins in a robust manner holds tremendous potential for advancing next-generation multiplexed biomarker discovery. However, methods must be …

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Increasing sustainability in palaeoproteomics by optimizing digestion times for large-scale archaeological bone analyses2024

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Probe-dependent Proximity Profiling (ProPPr) Uncovers Similarities and Differences in Phospho-Tau-Associated Proteomes Between Tauopathies2024

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Proteomic Analysis Reveals Trilaciclib-Induced Senescence2024

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Direct Relationship between Protein Expression and Progeny Yield of Herpes Simplex Virus 1 Unveils a Rate-limiting Step for Virus Production2024

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Neat plasma proteomics: getting the best out of the worst2024, ,

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diaPASEF Enables High-throughput Proteomic Analysis of Host Cell Proteins for Biopharmaceutical Process Development2024, ,

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PU.1 Eviction at Lymphocyte-Specific Chromatin Domains Mediates Glucocorticoid Response in Acute Lymphoblastic Leukemia2024

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Fragment ion intensity prediction improves the identification rate of non-tryptic peptides in timsTOF2024

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timsTOF HT Improves Protein Identification and Quantitative Reproducibility for Deep Unbiased Plasma Protein Biomarker Discovery2024, , ,

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Full Mass Range ΦSDM Orbitrap Mass Spectrometry for DIA Proteome Analysis2024, ,

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Single cell proteomics by mass spectrometry reveals deep epigenetic insight and new targets of a class specific histone deacetylase inhibitor2024, , , , ,

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Fully automated workflow for integrated sample digestion and Evotip loading enabling high-throughput clinical proteomics2023, ,

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Production in Nicotiana benthamiana of a thermotolerant glucose oxidase that shows enzybiotic activity against Escherichia coli and Staphylococcus aureus2023

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Exploring the benefits of differential mobility separation and SWATH data independent acquisition for complex proteomic sample analysis2023,

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Comparative proteomics analysis in different stages of urothelial bladder cancer for identification of potential biomarkers: highlighted role for antioxidant activity, 2023

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A complete and automated end-to-end sample preparation strategy for high-throughput and standardized proteomics with high sensitivity2023, ,

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Rapid and robust PTM peptide mapping for biologics with the Evosep One and the ZenoTOF 76002023, , ,

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Vacuum Insulated Probe Heated Electrospray Ionization Source Enhances Microflow Rate Chromatography Signals in the Bruker timsTOF Mass Spectrometer2023, , , ,

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Evotip Pure simplifies workflows with excellent reproducibility, storage and recovery2023The Evotip plays a vital role in the workflow of the Evosep One. It seamlessly integrates front-end sample preparation with liquid chromatography, facilitating efficient mass spectrometry analysis. Evotip Pure can be utilized for sensitive targeted assays, here illustrated in combination with the Agilent 6495C, ,

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Towards a Standardized Omics Platform with the 100 samples per day method2023This application note describes the 100 samples per day standard method. Get an overview of the method, the chromatographic performance and the results expected from Hela standard injections.

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Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth, 2023The Evosep One offers a diverse range of applications, utilizing its six standard methods. It caters to various use cases, spanning from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis., , , ,

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End-to-end, fully automated digestion protocol and Evotip Pure workflow on the Opentrons OT-22023The Evosep One provides a standardized and user-friendly platform that enables routine analysis of hundreds of proteomes per instru- ment per day, which has shifted the bottleneck for realizing high-throughput proteomics towards sample preparation. To address this, we have developed a fully automated Evotip loading protocol, based on a unique layered sandwich approach, which is …,

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Fully automated, rapid, robust sample loading on Evotip Pure with the Agilent AssayMAP Bravo2023Automation and robotics have revolutionized the field of proteomics, offering solutions to the growing need for streamlined workflows and efficient sample processing. In this context, automated loading of Evotips has emerged as a pivotal advancement in sample preparation workflows. The Evotip can now be loaded using an innovative and fully automated strategy with the Agilent …, ,

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Flexibility, speed, and throughput for high proteome coverage using Zeno SWATH data-independent acquisition (DIA) coupled with the Evosep One system2023, , , , , ,

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Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer2023, , , ,

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Chromatographic separation of peptides and proteins for characterization of proteomes2022, ,

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A complete, automated Opentrons OT-2 loading protocol for simplified sample loading of Evotip Pure2022, ,

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Rapid and robust PTM peptide mapping for biologics with the Evosep One and the SCIEX ZenoTOF 76002022, ,

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Synchro-PASEF allows precursor-specific fragment ion extraction and interference removal in data-independent acquisition2022,

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Extended detection of anabolics misuse in high-throughput doping controls with the Evosep One2022

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High-throughput mass spectrometry maps the sepsis plasma proteome and differences in response2022

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High-throughput quantitative proteomics using Zeno SWATH data-independent analysis (DIA) and the Evosep One system2022, , ,

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Rapid and in-depth coverage of the (phospho-)proteome with deep libraries and optimal window design for dia-PASEF2022, ,

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Using Targeted Liquid Chromatography-Tandem Mass Spectrometry to Rapidly Detect β-Lactam, Aminoglycoside, and Fluoroquinolone Resistance Mechanisms in Blood Cultures Growing E. coli or K. pneumoniae2022,

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ABPP-HT* – Deep meets fast for activity-based profiling of deubiquitylating enzymes using advanced DIA and DDA mass spectrometry methods2022

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Rapid and Accurate Detection of Aminoglycoside-Modifying Enzymes and 16S rRNA Methyltransferases by Targeted Liquid Chromatography-Tandem Mass, 2021This publication by the Dekker group describes the development of an accurate and rapid targeted LC-MS/MS assay based on parallel reaction monitoring for detection of the most prevalent aminoglycoside-modifying enzymes and 16S rRNA methyltransferases in Escherichia coli and Klebsiella pneumoniae that confer resistance to aminoglycosides.

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A blood atlas of COVID-19 defines hallmarks of disease severity and specificity, , 2021This publication is a major collaboration led by the Knight group at University of Oxford describing the hallmarks of Covid-19 severity and specificity. It is an integrative multi-omics analysis, where the Evosep One was used for measuring 340 plasma proteomes providing excellent patient stratification.

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Unleashing the power of DIA combined with short gradients using Evosep One, 2021This webinar is part of our Evosep webinar series and hosted by Nicolai Bache, Evosep. Florian Meier and Vadim Demichev discuss their use of the Evosep One, using novel DIA methodologies., ,

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Improve and enhance your proteome coverage with the Performance column2019We have developed an additional Performance column for the 60 and 100 samples per day methods (SPD) to address applications with high demands.,

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High sensitivity dia-PASEF proteomics with DIA-NN and FragPipe, 2021The Ralser and Nesvizhskii groups introduce the latest addition to the DIA-NN software with a neural network-based ion mobility module. DIA-NN was originally conceived to maximize the performance of fast proteomics experiments and here they demonstrate the identification of more than 5000 proteins from the 200 SPD method., ,

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Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers, , , 2021In this study, the Theis and Schiller groups at the Helmholtz Zentrum München, investigated the correspondence of cell state changes in diseased organs to peripheral protein signatures in pulmonary fibrosis patient cohorts. From plasma proteome profiling of more than 122 patients, they propose CRTAC1 protein levels in plasma as a novel biomarker.,

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SPIN – Species by Proteome Investigation, 2021This publication from the Olsen Group, introduces “Species by Proteome INvestigation” (SPIN), a proteomics workflow capable of querying over 150 mammalian species 200 times a day with the Evosep One. Genetic species determination is an indispensable tool in forensics, archaeology, ecology, and food authentication., ,

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ABPP-HT – high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context, 2020This publication from University of Oxford led by Adan Pinto-Fernandez, describes a new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics.

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diaPASEF: parallel accumulation–serial fragmentation combined with data-independent acquisition, 2020A collaboration led by the Mann group at the Max Planck Institute for Biochemistry, Münich describes the development of the latest addition to the PASEF technology. The diaPASEF workflow combines the advantages of DIA such as high degree of reproducibility and data completeness with the effective PASEF technology., ,

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High throughput proteome and phosphorpoteome sample processing coupled to fast gradient DIA, , , , , , , 2020High throughput workflows for proteomics, ,

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Optimizing data processing parameters for samples acquired on instruments using Advanced Peak Determination, 2018Improving ID rates with APD on Q Exactive HF-X

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New Orbitrap Exploris 480 Mass Spectrometer Coupled with Evosep One, , , 2020Brochure of Exploris 480 with Evosep data from piece no 1, ,

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High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry, , 2020BoxCar DIA,

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Improving proteome coverage and peptide identification rates in short LC gradients, , , , , 2020, ,

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High-throughput proteomics with Evosep One, , , , , 2020, ,

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Using Artificial Intelligence on Ultrafast LC-MSMS-DIA runs for Bacterial Identification in Urine, , 2019View the recording of our HUPO 2019 lunch seminar, where Florence Roux-Dalvai from Québec Research Center, Canada presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs.  , ,

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High-throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients, 2020diaPASEF app note  , , ,

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MaxQuant software for ion moiblity enhanced shotgun proteomics, , 2020MaxQuant for timsTOF analysis

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Plasma proteomics goes high-throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min, , 2019The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human blood plasma (blood plasma from 192 severe infection patients).

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Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform, , , , 2019In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard., ,

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timsTOF Pro with PASEF and Evosep One: Maximizing throughput, robustness and analytical depth for shotgun proteomics, 2018Read this application note to learn more about the timsTOF Pro with PASEF and the Evosep One for maximizing throughput, robustness and analytical depth for shotgun proteomics. Increased peak capacity via trapped ion mobility spectrometry allows the analysis of 200 samples/day, at formidable analytical depth, with unparalleled robustness., ,

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Development of mass spectrometry-based targeted assay for direct detection of novel SARS-CoV-2 coronavirus from clinical specimens, , , 2020This publication by the Akhilesh Pandey group describes a targeted FAIMS-PRM assay on an Orbitrap Exploris 480 MS. More than 700 nasopharyngeal swab samples were analyzed with high specificity and comparable sensitivity to the gold standard RT-PCR method for the diagnosis of SARS-CoV-2.,

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A Compact Quadrupole-Orbitrap Mass Spectrometer With Faims Interface Improves Proteome Coverage In Short Lc Gradients, , , , , , , , , 2020Test of Orbitrap Exploris with DIA and TMT, proteome and phospho., ,

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Accurate Detection Of The Four Most Prevalent Carbapenemases In E. Coli And K. Pneumoniae By High-Resolution Mass Spectrometry, 2019“Proof of concept” study showing the potential of targeted high resolution MS in detecting different resistance mechanisms. Accurate detection of KPC, OXA-48-like, NDM and VIM carbapenemases.

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High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition, , 2018High throughput LC-PRM to monitor the main protein components of AKT/mTOR signaling pathway., ,

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Fast Confirmatory Analysis of Rhuepos in Plasma for Horseracing Doping Control, , 2019This application note describes a rapid targeted analysis monitoring two tryptic rHuEPO peptides in plasma for horseracing doping control. The results illustrate the promising capabilities of the Evosep One innovative technology for the analysis of protein/peptide-based drugs in the context of drug testing.

Download

DOWNLOAD OUR MATERIAL

application note
Towards a Standardized Omics Platform with the 100 samples per day method
APPLICATION NOte 
THE PROTEOMICS DILEMMA – HIGH THROUGHPUT ANALYSIS VERSUS PROTEOME DEPTH
application note
FAST CONFIRMATORY ANALYSIS OF RHUEPOS IN PLASMA FOR HORSERACING DOPING CONTROL

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