The 30 samples per day method
STANDARD METHOD
The longest standard method
The 30 SPD method is the longest of our standard methods with a 44-minute gradient.
It is a popular choice for routine applications such as measuring proteome and post-translational modified peptides.
HIGHEST PROTEOME COVERAGE
This method provides the highest proteome coverage among our standard methods with close to 25,000 unique peptides identified in data-dependent acquisition mode.
The five standard methods
With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You device how fast you want to go.
Standard method
The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.
Standard method
Excellent sequencing speed with most peptides per minute among our standard methods.
Standard method
A great “in-between” method with run-to-run reproducibility of just one second.
Standard method
The longest method with a 44-minute gradient providing the best overall proteome coverage.
Explore more
Do you want to know how our users are already taking advantage of the 30 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.
| Title | Subject | Material | Year | Summary | Institute | Evosep method | MS instrumentation | Learn More |
|---|---|---|---|---|---|---|---|---|
| Seroconversion stages COVID19 into distinct pathophysiological states | Clinical research, Covid-19, Plasma | Publication | 2020 | In this study, researchers from University of Colorado led by Joaquin M. Espinosa, found highly variable seroconversion status among hospitalized Covid-19 patients. Their results support the existence of distinct pathophysiological states, with seroconversion status being potentially useful as a surrogate marker of underlying processes. | University of Colorado, United States. | 30 SPD | Bruker timsTOF Pro | |
| Development of a multiplexed targeted mass spectrometry assay for LRRK2-phosphorylated Rabs and Ser910/Ser935 biomarker sites | Clinical research, Parkinson's disease, Targeted workflow | Publication | 2020 | This publication from University of Dundee led by Dario R Alessi, describes the validation and development of a new, multiplexed targeted assay that enables the relative quantification of the key components of the LRRK2 pathway, defining the impact of LRRK2 inhibitors and Parkinson’s disease causing mutations. | University of Dundee, United States. Medical University of Vienna, Austria. | 30 SPD, 60 SPD | Thermo Orbitrap Exploris 480 | |
| High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry | DIA, Plasma, Technology | Poster | 2020 | BoxCar DIA | Max Planck Institute of Biochemistry, Martinsried, Germany | 100 SPD, 30 SPD | Thermo Orbitrap Exploris 480 | |
| Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time | DDA, Lung, Tissue, TMT | Video | 2019 | Comparison study with TMT, same coverage in shorter time | SickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada | 30 SPD, 60 SPD | Thermo Orbitrap Fusion Lumos | |
| Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development | Biopharma, DDA, HCP | Publication | 2020 | HCP analysis in Biopharma | Glaxo Smith Kline, Unites States | 30 SPD, 60 SPD | Thermo Orbitrap Fusion Lumos | |
| Characterization of glutathione proteome in CHO cells and its relationship with productivity and cholesterol synthesis | DDA, TMT | Publication | 2020 | Technical University of Denmark | 30 SPD | Thermo Q Exactive HF-X | ||
| An Ultra High-Throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer | Clinical research, DDA, Lung, Plasma, Serum, Tissue | Application note | 2020 | In this application note, the group of Emily Chen at the Thermo Fisher Precision Medicine Science Center describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard. | Thermo Fisher Scientific, United States | 30 SPD, 60 SPD | Thermo Orbitrap Exploris 240 | |
| Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform | Clinical research, DDA, Lung, Plasma, Serum | Application note | 2019 | In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard. | Thermo Fisher Scientific, United States | 100 SPD, 30 SPD, 60 SPD | Thermo Q Exactive HF-X | |
| Multi-level proteomics reveals host-perturbation strategies of SARS-CoV-2 and SARS-CoV | Clinical research, Covid-19, DIA, PTM | Publication | 2020 | This publication describes the molecular functions of viral proteins and their interactions with the host proteome of SARS-CoV-2. The impact of viral infection on the proteome and phosphoproteome were analyzed in a time-resolved manner by DIA. The analysis revealed key pathways perturbed during the infection identifying potential vulnerable points of SARS-CoV-2. | Max Planck Institute of Biochemistry, Martinsried, Germany | 30 SPD, 60 SPD | Thermo Q Exactive HF-X | |
| A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease | Clinical research, DIA, Liver, Plasma | Publication | 2020 | This publication from Matthias Mann groups in Copenhagen and Münich describes a paired liver biopsy and plasma proteomics study, which make use of Boxcar DIA scanning for a large clinical cohort of nearly 600 patients. They have developed a machine learning model based on their biomarker panel, which for the first time outperforms existing tests, … | Max Planck Institute of Biochemistry, Martinsried, Germany and Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark | 30 SPD | Thermo Q Exactive HF-X | |
| Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time | Offline fractionation, Tissue, TMT | Video | 2019 | Comparison study with TMT, same coverage in shorter time | SickKids Proteomics, Analytics, Robotics, and Chemical Biology Centre, Canada | 30 SPD, 60 SPD | Thermo Orbitrap Fusion Lumos |
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| Covalent Protein Painting Reveals Structural Changes In The Proteome In Alzheimer Disease | Alzheimer Disease, Brain, DDA, Tissue | Publication | 2020 | Development of Covalent Protein Painting (CPP), a structural proteomics approach to quantify changes in protein fold or altered protein-protein interaction for any protein in a proteome. CPP directly determines the relative surface accessibility of amino acid side chains by measuring the molar fraction of a chemical functionality that is accessible for chemical modification on the … | Scripps Research Institute, United States | 30 SPD | Thermo Orbitrap Fusion Lumos |
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