The 30 samples per day method

STANDARD METHOD

The longest standard method

The 30 SPD method is the longest of our standard methods with a 44-minute gradient.

It is a popular choice for routine applications such as measuring proteome and post-translational modified peptides.

HIGHEST PROTEOME COVERAGE

This method provides the highest proteome coverage among our standard methods with close to 25,000 unique peptides identified in data-dependent acquisition mode.

The Six standard methods

With the six standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You decide how fast you want to go. 

Standard method

The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.

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Standard method

Very high peak capacity per minute for high-throughput analysis.

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Standard method

Excellent sequencing speed with most peptides per minute among our standard methods.

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Standard method

A great “in-between” method with run-to-run reproducibility of just one second.

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Standard method

The most popular method with an excellent balance of throughput and depth.

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Standard method

The longest method with a 44-minute gradient providing the best overall proteome coverage.

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Explore more

Do you want to know how our users are already taking advantage of the 30 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.

TitleSubjectMaterialYearSummaryInstituteEvosep methodMS instrumentationLearn More
Towards routine proteome profiling of FFPE tissue: Insights from a 1,200 case pan-cancer study2024, ,

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Culturing Conditions Dictate the Composition and Pathways Enrichment of Human and Rat Perirenal Adipose-Derived Stromal Cells’ Secretomes2024

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Visfatin impact on the proteome of porcine luteal cells during implantation2024

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Nivolumab reaches brain lesions in patients with recurrent glioblastoma and induces T-cell activity and upregulation of checkpoint pathways2024

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Single cell proteomics by mass spectrometry reveals deep epigenetic insight into the actions of an orphan histone deacetylase inhibitor2024, , , ,

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Proteomic Characterisation of Placenta Tissue for Biomarker Discovery – Workflow Development and Application2024

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Engineering of TurboID-Wingless for the identification of Wingless interactors through in vivo proximity labelling2024,

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A novel hybrid high speed mass spectrometer allows rapid translation from biomarker candidates to targeted clinical tests using 15N labeled proteins2024, ,

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Hepatocellular RECK as a Critical Regulator of Metabolic Dysfunction-Associated Steatohepatitis Development2024

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Deregulation of cellulose synthesis by site-directed mutagenesis of cellulose synthase leads to heightened bacterial cellulose production2024

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Broad proteomics analysis of seeding-induced aggregation of α-synuclein in M83 neurons reveals remodeling of proteostasis mechanisms that might contribute to Parkinson’s disease pathogenesis2024

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A proteogenomic atlas of the human neural retina2024

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Effect of hypercholesterolemia on circulating and cardiomyocyte-derived extracellular vesicles2024

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GLP-1-directed NMDA receptor antagonism for obesity treatment2024

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The balance between antiviral and antibacterial responses during M. Tuberculosis infection is regulated by the ubiquitin ligase CBL2024, ,

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Longitudinal Fluctuations in Protein Concentrations and Higher-Order Structures in the Plasma Proteome of Kidney Failure Patients Subjected to a Kidney Transplant2024

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Optimization of protocols for blood-derived extracellular vesicles for studies in liver diseases2024

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Urinary Proteomics and Outcomes in Heart Failure With Preserved Ejection Fraction2024

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Spatial Proteomics Reveals Alcohol-Induced Damages to the Crypts and Villi of the Mouse Small Intestine2024

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Global organization of phenylpropanoid and anthocyanin pathways revealed by proximity labeling of trans-cinnamic acid 4-hydroxylase (CYP73A412) in Petunia inflata petal protoplasts2024

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Enrichment, Characterization, and Proteomic Profiling of Small Extracellular Vesicles Derived from Human Limbal Mesenchymal Stromal Cells and Melanocytes2024

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Exploring the role of production and release of proteins for microbial interactions in kombucha2024

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Extracellular vesicles derived CXCL4 is a candidate serum tumor biomarker for colorectal cancer2024

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Cheap, Robust and Versatile Two-Dimensional Chromatography system for Proteomics of Nanogram Scale Samples2024

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RNA damage compartmentalization by DHX9 stress granules2024

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Theileria parasites sequester host eIF5A to escape elimination by host-mediated autophagy2024

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Characterization of preovulatory follicular fluid secretome and its effects on equine oocytes during in vitro maturation2024

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Personalized phosphoproteomics of skeletal muscle insulin resistance and exercise links MINDY1 to insulin action2024

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Proteomic profiling of IDH-wildtype Glioblastoma Tissue and Serum uncovers prognostic Subtypes and Marker Candidates2024

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TIMAHAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics2024

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Polydopamine nanoparticles as a non-pharmaceutical tool in the treatment of fatty liver disease2024

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diaPASEF Enables High-throughput Proteomic Analysis of Host Cell Proteins for Biopharmaceutical Process Development2024, ,

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The UFM1 E3 ligase recognizes and releases 60S ribosomes from ER translocons2024

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Automated high-throughput biological sex identification from archaeological human dental enamel using targeted proteomics2024,

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Comparative proteomic analysis of the changes in mare milk associated with different lactation stages and management systems2024

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Personalized Molecular Signatures of Insulin Resistance and Type 2 Diabetes2024

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STREAMLINED PROTEOME-WIDE IDENTIFICATION OF DRUG TARGETS INDICATES ORGAN-SPECIFIC ENGAGEMENT2024

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Proximity labeling proteomics reveals Kv1.3 potassium channel immune interactors in microglia2024,

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Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition2024,

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The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells2024

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Implementation of pupylation-based proximity labelling in plant biology reveals regulatory factors in cellulose biosynthesis2024

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Mechanism of chaperone coordination during cotranslational protein folding in bacteria2024

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timsTOF HT Improves Protein Identification and Quantitative Reproducibility for Deep Unbiased Plasma Protein Biomarker Discovery2024, , ,

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Kinase inhibitor pulldown assay (KiP) for clinical proteomics2024

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Cerebrospinal fluid shotgun proteomics identifies distinct proteomic patterns in cerebral amyloid angiopathy rodent models and human patients2024

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Direct translation of incoming retroviral genomes2024

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Single cell proteomics by mass spectrometry reveals deep epigenetic insight and new targets of a class specific histone deacetylase inhibitor2024, , , , ,

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A biotin targeting chimera (BioTAC) system to map small molecule interactomes in situ2023

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Proteomic comparison between non‐purified cerebrospinal fluid and cerebrospinal fluid‐derived extracellular vesicles from patients with Alzheimer’s, Parkinson’s and Lewy body dementia2023

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Fully automated workflow for integrated sample digestion and Evotip loading enabling high-throughput clinical proteomics2023, ,

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IMBAS-MS discovers organ-specific HLA peptide patterns in plasma2023, , ,

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Multicenter Collaborative Study to Optimize Mass Spectrometry Workflows of Clinical Specimens2023,

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High-throughput proteomics uncovers exercise training and type 2 diabetes–induced changes in human white adipose tissue2023

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BioE3 identifies specific substrates of ubiquitin E3 ligases2023

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Mapping cardiac remodeling in chronic kidney disease2023

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Antitumor activity of AZD0754, a dnTGFβRII-armored, STEAP2-targeted CAR-T cell therapy, in prostate cancer, , , 2023

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Sensitive and Specific Global Cell Surface N-Glycoproteomics Shows Profound Differences Between Glycosylation Sites and Subcellular Components, , 2023

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Dynamic extracellular proximal interaction profiling reveals Low-Density Lipoprotein Receptor as a new Epidermal Growth Factor signaling pathway component2023,

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Mass spectrometry quantifies target engagement for a KRASG12C inhibitor in FFPE tumor tissue, , 2023

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Interplay between Cultured Human Osteoblastic and Skeletal Muscle Cells: Effects of Conditioned Media on Glucose and Fatty Acid Metabolism2023

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A chemoproteomic platform for selective deubiquitinase inhibitor discovery, 2023,

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Species-specific metabolic reprogramming in human and mouse microglia during inflammatory pathway induction, 2023

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Autoantibody binding and unique enzyme-substrate intermediate conformation of human transglutaminase 32023

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Direct isolation of small extracellular vesicles from human blood using viscoelastic microfluidics, , , 2023

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Spatial single-cell mass spectrometry defines zonation of the hepatocyte proteome, 2023,

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MassSpecPreppy—An end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications2023

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Heterogeneity of radial spokes structural components and associated enzymes in Tetrahymena cilia, 2023

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A dynamic bias in chromatin protein deposition at G-quadruplex sites, 2023

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A micro-flow, high-pH, reversed-phase peptide fractionation and collection system for targeted and in-depth proteomics of low-abundance proteins in limiting samples, 2023

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Selective targeting of IL2Rbg combined with radiotherapy triggers CD8- and NK-mediated immunity, abrogating metastasis in HNSCC, 2023,

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Deep and fast label-free Dynamic Organellar Mapping, , 2023,

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Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases2023

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An unconventional TOG domain is required for CLASP localization, 2023

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Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae, , 2023,

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Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) in C. elegans embryos, , , 2023

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Fatty acids abrogate the growth-suppressive effects induced by inhibition of cholesterol, 2023

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Cofilin-Driven Nuclear Deformation Drives Dendritic Cell Migration through the Extracellular Matrix, , , 2023,

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A seven-transmembrane methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenases, , , 2023,

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Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling, , , , , 2023, , ,

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A complete and automated end-to-end sample preparation strategy for high-throughput and standardized proteomics with high sensitivity2023, ,

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Altered intratumoral immune composition after Nivolumab treatment in patients with recurrent glioblastoma2023

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Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their function2023

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Using In Vivo Intact Structure for System-wide Quantitative Analysis of Changes in Proteins2023

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DIS3 ribonuclease prevents the cytoplasmic accumulation of lncRNAs carrying non-canonical ORFs, which represent a source of cancer immunopeptides2023

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The protein and miRNA profile of plasma extracellular vesicles (EVs) can distinguish feline mammary adenocarcinoma patients from healthy feline controls2023

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Co-administration of angiotensin II and simvastatin triggers kidney injury upon heme oxygenase-1 deficiency2023

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Vacuum Insulated Probe Heated Electrospray Ionization Source Enhances Microflow Rate Chromatography Signals in the Bruker timsTOF Mass Spectrometer2023, , , ,

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Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b2023

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Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth, 2023The Evosep One offers a diverse range of applications, utilizing its six standard methods. It caters to various use cases, spanning from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis., , , ,

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Proteomic Analysis of Mesenchymal Stem Cells and Monocyte Co-Cultures Exposed to a Bioactive Silica-Based Sol–Gel Coating2023

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In depth profiling of the cancer proteome from the flowthrough of standard RNA-preparation kits for precision oncology2023

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Matrix Gla Protein drives stemness and tumor initiation in ovarian cancer2023

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A desert Chlorella sp. that thrives at extreme high-light intensities using a unique photoinhibition protection mechanism2023

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Flexibility, speed, and throughput for high proteome coverage using Zeno SWATH data-independent acquisition (DIA) coupled with the Evosep One system2023, , , , , ,

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Suspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer’s Disease Mouse Model2023

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Dynamic antagonism between key repressive pathways maintains the placental epigenome2023

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Blue Native PAGE–Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes: Detection of a Cerebellar α1/α6-Subunits Containing γ-Aminobutyric Acid Type A Receptor Subtype2023

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Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays2023

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Towards a Standardized Omics Platform with the 30 samples per day method2023This application note describes the 30 samples per day standard method. Get an overview of the method, the chromatographic performance and the results expected from Hela standard injections.

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Luminescence-based screening for extracellular vesicle release modulators reveals a role for PI4KIIIβ in exosome biogenesis upon lysosome inhibition2023

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Mechanisms of Nuclear Pore Complex disassembly by the mitotic Polo Like Kinase 1 (PLK-1) in C. elegans embryos2023

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An Electrophysiological and Proteomic Analysis of the Effects of the Superoxide Dismutase Mimetic, MnTMPyP, on Synaptic Signalling Post-Ischemia in Isolated Rat Hippocampal Slices2023

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No-stain protein labeling as a potential normalization marker for small extracellular vesicle proteins2023

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Mesenchyme-derived vertebrate lonesome kinase controls lung organogenesis by altering the matrisome2023

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Vacuum Insulated Probe Heated ElectroSpray Ionization source (VIP-HESI) enhances micro flow rate chromatography signals in the Bruker timsTOF mass spectrometer2023, , , ,

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Extracellular Vesicles Isolated from Plasma of Multiple Myeloma Patients Treated with Daratumumab Express CD38, PD-L1, and the Complement Inhibitory Proteins CD55 and CD592022

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Systematic Adaptation of Bacillus licheniformis to 2-Phenylethanol Stress2023

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A chemoproteomic platform for reactive fragment profiling against the deubiquitinases2023,

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Exploiting Multi-Omics Profiling and Systems Biology to Investigate Functions of TOMM342023

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β-oxidation–polyhydroxyalkanoates synthesis relationship in Pseudomonas putida KT2440 revisited2023

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Extending the Range of SLIM-Labeling Applications: From Human Cell Lines in Culture to Caenorhabditis elegans Whole-Organism Labeling, 2023

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Quantitative multi-organ proteomics of fatal COVID-19 uncovers tissue-specific effects beyond inflammation2023

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Proteomic evaluation of human osteoblast responses to titanium implants over time2022

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MS-DAP Platform for Downstream Data Analysis of Label-Free Proteomics Uncovers Optimal Workflows in Benchmark Data Sets and Increased Sensitivity in Analysis of Alzheimer’s Biomarker Data2022

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Temporal phosphoproteomics reveals WEE1-dependent control of 53BP1 pathway2022

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Characterization of TGF-β signaling in a human organotypic skin model reveals that loss of TGF-βRII induces invasive tissue growth2022

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Correlation between biological responses in vitro and in vivo to Ca-doped sol-gel coatings assessed using proteomic analysis2022

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Kinase Inhibitor Pulldown Assay (KiP) for Clinical Proteomics2022

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2-O-Methylation of the second transcribed nucleotide within the mRNA 5 cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion2022

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PKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074, and THR1075) within the COR GTPase domain2022

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Cellular proteomic profiling using proximity labelling by TurboID-NES in microglial and neuronal cell lines2022

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The spike of SARS-CoV-2 promotes metabolic rewiring in hepatocytes2022

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The addition of FAIMS increases targeted proteomics sensitivity from FFPE tumor biopsies2022

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Widespread hydroxylation of unstructured lysine-rich protein domains by JMJD62022

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High throughput and high confidence sequence variant analysis in therapeutic antibodies using Evosep One liquid chromatography tandem mass spectrometry with synthetic heavy peptides2022

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Osteocytes Serve as a Reservoir for Intracellular Persisting Staphylococcus aureus Due to the Lack of Defense Mechanisms2022

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Neddylation tunes peripheral blood mononuclear cells immune response in COVID-19 patients2022

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High-throughput quantitative proteomics using Zeno SWATH data-independent analysis (DIA) and the Evosep One system2022, , ,

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Rapid and in-depth coverage of the (phospho-)proteome with deep libraries and optimal window design for dia-PASEF2022, ,

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Brain proteome profling implicates the complement and coagulation cascade in multiple system atrophy brain pathology2022

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Proteomic phenotype of cerebral organoids derived from autism spectrum disorder patients reveal disrupted energy metabolism, cellular components, and biological processes2022

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Inflammation-induced downregulation of succinate dehydrogenase regulates adrenocortical function2022

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Integrative analysis of non-small cell lung cancer patient-derived xenografts identifies distinct proteotypes associated with patient outcomes2022

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DebaryOmics: an integrative –omics study to understand the halophilic behaviour of Debaryomyces hansenii2022

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Comprehensive proteomics with 8.000 proteins identified with DIA-PASEF using the 30 samples per day method2022

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Improve and enhance your proteome coverage with the performance column for our long methods2022,

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Toxoplasma gondii ROP1 subverts murine and human innate immune restriction2022

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Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase2021This publication by the Costa group describes the mechanism whereby the Dbf4 dependent kinase selectively targets fully loaded MCM double hexamers on the path to helicase activation, and uncover the mechanism whereby the checkpoint kinase Rad53 blocks this function.

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Hyperinflammatory environment drives dysfunctional myeloid cell effector response to bacterial challenge in COVID-192022This publication by the Zinkernagel group, assess the influence of COVID-19 plasma hypercytokinemia on the functional responses of myeloid immune cells upon bacterial challenges from acute-phase COVID-19 patients and their corresponding recovery-phase. We show that a severe hypercytokinemia status in COVID-19 patients correlates with the development of bacterial superinfections.

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Data independent acquisition enables deep and fast label-free dynamic organellar mapping2021This publication by the Borner group, describes a DIA workflow to establish organellar maps, which combines cell fractionation and shotgun proteomics into a profiling analysis of subcellular localization. They apply this to HeLa cells and investigate changes in response to starvation/disruption of lysosomal pH.,

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Plasma extracellular vesicles in people living with HIV and type 2 diabetes are related to microbial translocation and cardiovascular risk, , , 2021This publication, led by the Trøseid group at Oslo University Hospital investigates the potential role of plasma extracellular vesicles (EVs) in relation to gut microbiota alterations and low-grade endotoxemia in HIV and type 2 diabetes.

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DebaryOmics: an integrative –omics study to understand the halophilic behaviour of Debaryomyces hansenii, , , 2021This publication by the Martinez group at the Technical University of Denmark, presents the first global phosphoproteomics analysis in Debaryomyces hansenii, a non-conventional yeast considered to be a well-suited option for a number of different industrial bioprocesses.

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Evaluation of Disposable Trap Column nanoLC–FAIMS–MS/MS for the Proteomic Analysis of FFPE Tissue, , , 2021This publication by the Kuster group presents a robust workflow to support the analysis of large cohorts of patient samples using formalin-fixed paraffin-embedded FFPE tissue. They make use of online fractionation by FAIMS requiring 50% less sample than conventional high pH fractionation., ,

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High-Throughput Drug Discovery with the Evosep One2021In this webinar we will look into how the Evosep One with high-throughput and sensitivity is a good fit when developing new drug applications or biologics license applications.

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Plasma Proteomics with the Evosep One, , 2021In this webinar you will learn how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.,

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A deeper look at carrier proteome effects for single-cell proteomics, , 2021This publication by the Olsen group at University of Copenhagen describes the carrier proteome effects in single cell proteomics with mixed species TMTpro-labeled samples. Using the 30 SPD method, they show that quantitative precision and signal intensity are limited at high carrier levels, hindering the recognition of regulated proteins.

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Time evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA, , 2021This publication, led by the Esbjörner group at the Chalmers University of Technology in Gothenburg, Sweden investigates the uptake and delivery of mRNA formulated into MC3 lipid nanoparticles focusing on the role of serum proteins in driving the temporal evolution of PEG-shedding reactions and protein coronation events.

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PD-L1 Overexpression, SWI/SNF Complex Deregulation, and Profound Transcriptomic Changes Characterize Cancer-Dependent Exhaustion of Persistently Activated CD4+ T Cells, , 2021This publication from the Sarnowska group at Maria Sklodowska-Curie National Research Institute of Oncology in Poland investigates the mechanisms of immune response during continuous stimulation of tumor-infiltrating lymphocytes by tumors. They use the Evosep One to measure the secreted proteins from restimulated CD4+ T cells grown with cancer cells.

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A personalized mass spectrometry-based assay to monitor M-protein in multiple myeloma patients (EasyM), , , 2021This publication by the Trudel group describes the development of a non-invasive MS-based assay, called EasyM, which is used to assess minimal residual disease, a measure of depth of response to treatment, which has become an important parameter in assessing the disease burden in multiple myeloma., ,

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Exploitation of the Secretory Pathway by SARS-CoV-2 Envelope, , 2021This publication by the Carlton group investigates the mechanism, by which Coronaviral Eenvelope proteins achieves its steady state localization to the Endoplasmic Reticulum Golgi Intermediate Compartment. They use proximity biotinylation to define the vicinal proteome of wild type and ER-restricted versions of Envelope.

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Mass Spectrometry Provides a Highly Sensitive Noninvasive Means of Sequencing and Tracking M-Protein in the Blood of Multiple Myeloma Patients, , , 2021The amino acid sequence of the M-protein for multiple myeloma is unique compared to the polyclonal antibodies in patients’ blood. This publication from Rapid Novor describes a targeted MS/MS assay to detect and quantify the unique M-protein sequence in serum samples from multiple myeloma patients.

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An ultrasensitive method for analysis of viral spike N-glycoforms, , 2021This publication from the Yates group describes a new ultrasensitive “single-pot” method applicable for analysis of site-specific glycosylation of any glycoprotein and characterizing glycosylation of viral spike proteins and potential vaccines.,

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Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures, , , , , 2021In this publication, the Olsen group highlights the potential of proteomics to dissect alterations in cellular signaling and identify druggable pathways in cancer. They identify protein kinase C delta (PKCδ) inhibition as a strategy to overcome resistance to Notch1 inhibition in T-cell acute lymphoblastic leukemia.,

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Co-translational assembly and localized translation of nucleoporins in nuclear pore complex biogenesis, 2021In this study, the Palancade group provides the complete depiction of the co-translational events involved in the biogenesis of a large multiprotein assembly, the nuclear pore complex (NPC) in yeast. Their data reveal that distinct, spatially segregated modes of co-translational interactions foster the ordered assembly of NPC subunits.

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Hemorrhage and saline resuscitation are associated with epigenetic and proteomic reprogramming in the rat lung, , , 2021In this study, the Sillesen group investigates the alteration of the proteome in the context of hemorrhage and saline resuscitation in trauma patients. They used rat lungs as a model and pooled 10 rats in one combined TMT experiment subjected to high pH offline fractionation.

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Malaria Parasite Schizont Egress Antigen-1 Plays an Essential Role in Nuclear Segregation during Schizogony, , 2021This publication by the Blackman group describes the role of SEA1, a suggested malaria vaccine antigen candidate. They conclude that SEA1 does not play a direct mechanistic role in egress to invade new erythrocytes but instead is an essential blood-stage protein that plays an important role in orchestrating the correct partitioning of DNA into merozoites.

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The COVIDome Explorer Researcher Portal, , , 2021Researchers from University of Colorado led by Joaquin M. Espinosa, have created a user-friendly researcher portal enabling easy access and real-time analysis of matched multi-omics datasets for COVID-19, termed the COVIDome Explorer. They illustrate the use through a multi-omics analysis of biosignatures associated with C-reactive protein (CRP), an established marker of poor prognosis in COVID-19.

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The Host Interactome of Spike Expands the Tropism of SARS-CoV-2, , 2021This publication from the Yates lab, investigates the host interactome determining whether a SARS-CoV-2 infection is productive. They find an “S2 only” dependent, alternative infection of additional cell types with SARS-CoV-2 may impact vaccination strategies and provide a molecular explanation for a severe or prolonged progression of disease in select COVID-19 patients.

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Basement membrane stiffness determines metastases formation, , , 2021In this study, the Erler group from the BRIC Institute at University of Copenhagen, Denmark identifies Net4 as a key regulator of basement membrane stiffness, a special type of extracellular matrix, which presents the major barrier cancer cells have to overcome multiple times to form metastases.

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Interactions of Viral Proteins from Pathogenic and Low or Non-Pathogenic Orthohantaviruses with Human Type I Interferon Signaling, 2021A collaboration led by the Ermonval group from Institute Pasteur describes the interactions of structural and non-structural proteins of pathogenic and low or non-pathogenic orthohantaviruses with human type I Interferon signaling. They suggest that the activation of IFN-I is probably not the only antiviral pathway to be counteracted by orthohantaviruses.

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Seroconversion stages COVID19 into distinct pathophysiological states, , 2020In this study, researchers from University of Colorado led by Joaquin M. Espinosa, found highly variable seroconversion status among hospitalized Covid-19 patients. Their results support the existence of distinct pathophysiological states, with seroconversion status being potentially useful as a surrogate marker of underlying processes.

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Development of a multiplexed targeted mass spectrometry assay for LRRK2-phosphorylated Rabs and Ser910/Ser935 biomarker sites, , 2020This publication from University of Dundee led by Dario R Alessi, describes the validation and development of a new, multiplexed targeted assay that enables the relative quantification of the key components of the LRRK2 pathway, defining the impact of LRRK2 inhibitors and Parkinson’s disease causing mutations.,

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High dynamic range proteome analysis with BoxCar DIA and super-resolution Orbitrap mass spectrometry, , 2020BoxCar DIA,

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Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development, , 2020HCP analysis in Biopharma,

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Characterization of glutathione proteome in CHO cells and its relationship with productivity and cholesterol synthesis, 2020

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An Ultra High-Throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer, , , , , 2020In this application note, the group of Emily Chen at the Thermo Fisher Precision Medicine Science Center describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard.,

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Scalable and Automated Plasma Workflow Based on the Thermo Scientific Q Exactive HF-X MS Platform, , , , 2019In this application note, the group of Emily Chen at PMSC describes a high-throughput plasma and serum proteomics analysis workflow for large population cohort studies that utilizes a standardized sample preparation method, high-throughput data acquisition, and easy to implement QC standard., ,

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Multi-level proteomics reveals host-perturbation strategies of SARS-CoV-2 and SARS-CoV, , , 2020This publication describes the molecular functions of viral proteins and their interactions with the host proteome of SARS-CoV-2. The impact of viral infection on the proteome and phosphoproteome were analyzed in a time-resolved manner by DIA. The analysis revealed key pathways perturbed during the infection identifying potential vulnerable points of SARS-CoV-2.,

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A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease, , , 2020This publication from Matthias Mann groups in Copenhagen and Münich describes a paired liver biopsy and plasma proteomics study, which make use of Boxcar DIA scanning for a large clinical cohort of nearly 600 patients. They have developed a machine learning model based on their biomarker panel, which for the first time outperforms existing tests, …

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Evosep One Enables Robust Deep Proteome Coverage Using Tandem Mass Tags While Significantly Reducing Instrument Time, , 2019Comparison study with TMT, same coverage in shorter time,

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Covalent Protein Painting Reveals Structural Changes In The Proteome In Alzheimer Disease, , , , 2020Development of Covalent Protein Painting (CPP), a structural proteomics approach to quantify changes in protein fold or altered protein-protein interaction for any protein in a proteome. CPP directly determines the relative surface accessibility of amino acid side chains by measuring the molar fraction of a chemical functionality that is accessible for chemical modification on the …

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Towards a Standardized Omics Platform with the 30 samples per day method
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