Evosep One

Product information

Evosep One PRODUCT INFORMATION

Get a better understanding of the applications and technology of Evosep One, a separation tool designed for clinical omics.

Application Note: Improve and enhance your proteome coverage with the performance column

We have developed a new Performance column for the 60 and 100 samples-per-day methods to address applications with high demands  The boost in sensitivity is particularly noticeable for lower loads, where peptide and protein coverage is improved with up to 100%.

Standard methods
Application Notes: 30spd, 60spd, 100spd, 200spd, 300spd

The five standard methods for the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis.

Application Note: THE PROTEOMICS DILEMMA – HIGH THROUGHPUT ANALYSIS VERSUS PROTEOME DEPTH

With the five standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis.

EVOSEP+
Application Note: MORE PROTEOME COVERAGE IN A SINGLE EXPERIMENT WITH THE EXTENDED METHOD

The Extended method is the ideal choice for maximum proteome coverage in a single experiment, which is preferred, Especially when the amount of starting material is limited.

Application Note: Fast and reproducible phosphoproteomics
using MagReSyn® Amine and Ti-IMAC HP magnetic beads and the Evosep One

Speed up your phosphoproteomics studies with an efficient and reproducible workflow using magnetic beads and the Evosep One.

Authors: Ana Martínez-Val, Dorte B. Bekker-Jensen, Sophia Steigerwald, Stoyan toychev, Isak Gerber,Justin Jordaan, Nicolai Bache, Jesper V. Olsen

Application Note: Fast confirmatory analysis of rHuEPOs in
plasma for horseracing doping control

Fast confirmatory analysis of rHuEPOs in plasma for horseracing doping control by coupling Evosep One & Q-Exactive HF.

Authors: Vivian Delcourt, Patrice Garcia, Benjamin Chabot, Mylène Caroff, Nicolai Bache, Marie-Agnès Popot and Ludovic Bailly-Chouriberry

Tech Note: EVOSEP ONE: A GRADIENT OFF-SET FOCUSING HPLC INSTRUMENT FOR ROBUST AND HIGH THROUGHPUT PROTEOMICS

Evosep One has been designed specifically to address and eliminate the prevalent challenges associated with throughput and robustness of nano-flow LC-MS workflows while maintaining sufficient sensitivity and resolving power for clinical omics applications.

Authors: Nicolai Bache, Ole Hoerning, Lasse Falkenby and Ole Vorm

Evosep One Field Test at the max-planck institute

Implementing the Evosep One – a ‘gradient off-set focusing HPLC instrument’– into the workflow at the Max-Planck Institute removed a major hurdle to establishing proteomics as a standard diagnostic method.

Application note: GRADIENT OFF-SET FOCUSING HPLC INSTRUMENT FOR ROBUST AND HIGH THROUGHPUT CLINICAL PROTEOMICS

Until now, available separation technology has severely limited throughput and robustness but the Evosep One dramatically increases robustness and sample throughput while maintaining the sensitivity of current nanoflow LC instruments.

Authors: Philipp Geyer,  Nicolai Bache, Sophia Doll, Peter Treit, Florian Meier, Matthias Mann

Brochure: Evosep One – A Separation Tool Designed for Clinical Omics

Evosep One is designed for sensitivity, throughput, and robustness – tailored for large clinical studies. Learn more about our instrument Evosep One, a separation tool designed for clinical omics.

Sample loading protocol for evotips

Evotips are disposable, purification and loading trap columns. Evotips fit most standard pipettes and commercial robotic systems. Using Evotips results in simplified workflows that removes several sample handling steps and reduces injection cycle overheads. 

Introducing Evosep One 

The Evosep One is an optimized front-end to a mass spectrometer for large-cohort experiments. Watch the video to get a quick overview of the instrument.

Did you not find what you were looking for?

If you need additional Evosep One product information, do not hesitate to contact us with any questions or comments you might have at sales@evosep.com.