We recommend loading 20ul of sample, but volume can be changed if the loading protocol is adapted accordingly (higher volume => Longer centrifugation time, lower volume => shorter centrifugation time)
No, once the sample has been eluted from the Evotip, the plastic will be slightly deformed and cannot be used again. Doing so, will void instrument warranty.
No, the Evosep One is accurately designed for a perfect low-pressure, leak-free fit with Evotips. Using other pipette tips will void instrument warranty.
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