The 200 samples per day method
STANDARD METHOD
HIGH-THROUGHPUT ANALYSIS
The 200 SPD method is well suited for routine high-throughput applications. The timing and chromatographic performance is maximized to deliver an effective gradient elution of 82% and narrow peaks with FWHM of 1.7 seconds on average.
EXCELLENT SEQUENCING SPEED WITH MOST PEPTIDES PER MINUTE
Good chromatographic quality is essential, and especially to fully employ the high MS/MS scan rates on modern mass spectrometers.
The 200 SPD method provides excellent sequencing speed with most peptides per minute among our standard methods. This translates to a high number of proteins identified per minute.
Maximizing throughput, robustness and analytical depth for shotgun proteomics with the timsTOF Pro
This application note describes the timsTOF PASEF workflow, which enables sequencing speed of more than 120 Hz, high sensitivity and robustness as well as an additional dimension of separation (TIMS) for improved peak capacity. More than 1400 protein groups from 50 ng of HeLa digest were identified with the 200SPD method, all with outstanding robustness and reproducibility.
SENSITIVE ANALYSIS WITH FAIMS Pro coupled to an orbitrap exploris 480 ms
In this publication the Olsen Group from the Novo Nordisk Foundation Center for Protein Research, Denmark, showcase the sensitivity of the FAIMS Pro device on an Orbitrap Exploris 480 MS by using the 200 SPD method.
They analyzed 5 ng of tryptic peptides equivalent to approximately 30-50 HeLa cells. By using single compensation voltage (CV) of -50, they were able to identify more than 1000 proteins. FAIMS enhanced the reproducibility of DIA quantification, based on the number of proteins quantified with coefficient of variation below 20%.
USING ARTIFICIAL INTELLIGENCE ON ULTRAFAST LC-MS/MS DIA RUNS FOR BACTERIAL IDENTIFICATION IN URINE
In this seminar, Florence Roux-Dalvai presents a new strategy for bacterial species identification in urinary tract infection using artificial intelligence on ultrafast LCMS DIA runs with 200 samples per day.
The work is a collaboration between Québec Research Center, Canada, Thermo Scientific and Evosep Biosystems.
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The six standard methods
With the six standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You decide how fast you want to go.
Standard method
The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.
Standard method
Excellent sequencing speed with most peptides per minute among our standard methods.
Standard method
A great “in-between” method with run-to-run reproducibility of just one second.
Standard method
The most popular method with an excellent balance of throughput and depth.
Standard method
The longest method with a 44-minute gradient providing the best overall proteome coverage.
Explore more
Do you want to know how our users are already taking advantage of the 200 Samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.
DOWNLOAD OUR MATERIAL
application note
Towards a Standardized Omics Platform with the 200 samples per day method
APPLICATION NOte
THE PROTEOMICS DILEMMA – HIGH THROUGHPUT ANALYSIS VERSUS PROTEOME DEPTH
BROCHURE
columns and emitters overview
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