The 60 samples per day method
STANDARD METHOD
More than 4000 proteins in 21 minutes
The 60 SPD method is one of our most popular standard methods as it provides an excellent balance between throughput and depth.
The method is ideal for deep fractionated proteomes as well as single-shot analyses, where more than 4000 proteins are routinely identified with DIA.
QUANTITATIVE RAT ORGAN PROTEIN ATLAS BY TMT AND dia-lfq
In this publication, the Olsen Group from the Novo Nordisk Foundation Center for Protein Research, Denmark, perform a comprehensive rat tissue specific protein atlas. They compare tandem mass tags (TMT11-plex) with DIA-based label-free quantification of 12 tissues covering the major mammalian organs from three individual male rats.
The spectral library generated, represents 213,000 unique peptides covering 12,909 protein-coding genes and should represent a resource for the proteomics community.
Unsupervised hierarchical clustering of relative protein abundances across tissues reveal very similar protein expression patterns between DIA-FAIMS and TMT experiments, suggesting that both quantification strategies provides very similar biological insights.
UNLEASHING THE TRUE POWER OF DIA ACQUISITION WITH SHORT GRADIENTS
In this case study, Ben C. Collins from Queen’s University, Belfast and Ting Huang, Northeastern University, Boston discuss the importance of fast and standardized workflows
Analysis speed can be increased by one order of magnitude with the Evosep One, reducing analysis time from roughly 10 days to a little more than 1 day without significant loss of information at the protein complex level. This enables novel applications for profiling proteome organization dynamics.
Data courtesy: Moritz Heusel and Isabell Bludau, ETH Zürich
FAST AND REPRODUCIBLE PHOSPHOPROTEOMICS
This application note describes an automated workflow for protein digestion as well as enrichment of phosphopeptides using MagReSyn magnetic beads for the entire workflow.
By employing a streamlined and automated workflow for phosphopeptide enrichment in combination with fast and robust chromatographic performance delivered by the Evosep One, we offer a workflow for routine use in large clinical studies, which can also be easily extended to tissue samples.
The use of automation in the entire workflow eliminates the hands-on challenges and ensures excellent reproducibility when handling a large number of samples.
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Discover how Evosep One simplifies workflows and supports labs of all sizes.
The six standard methods
With the six standard methods, the Evosep One covers a range of use cases from comprehensive proteome analysis with fractionation strategies to ultra high-throughput single-shot analysis. You decide how fast you want to go.
Standard method
The highest peak capacity per minute with our fastest method for ultra-high throughput analysis.
Standard method
Excellent sequencing speed with most peptides per minute among our standard methods.
Standard method
A great “in-between” method with run-to-run reproducibility of just one second.
Standard method
The most popular method with an excellent balance of throughput and depth.
Standard method
The longest method with a 44-minute gradient providing the best overall proteome coverage.
DOWNLOAD OUR MATERIAL
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Rapid and robust PTM peptide mapping for biologics with the Evosep One and the SCIEX ZenoTOF 7600
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Towards a Standardized Omics Platform with the 60 samples per day method
APPLICATION NOte
THE PROTEOMICS DILEMMA – HIGH THROUGHPUT ANALYSIS VERSUS PROTEOME DEPTH
CASE STUDY
Unleashing the true power of DIA/SWATH data acquisition with short gradients
application note
Fast and reproducible phosphoproteomics using MagReSyn® Amine and Ti-IMAC HP magnetic beads and the Evosep One
Explore more
Do you want to know how our users are already taking advantage of the 60 samples per day method? Find all publications from users and collaboration partners here. Visit our Literature Room for a full overview.
Want to know more?
Our team is ready to help you find the method fitting your research need. Fill out our contact form and we will get back to you as soon as possible
